Mills P R, Sreenivasaprasad S, Brown A E
Department of Mycology and Plant Pathology, Queen's University of Belfast, UK.
FEMS Microbiol Lett. 1992 Nov 1;77(1-3):137-43. doi: 10.1016/0378-1097(92)90145-e.
An oligonucleotide primer (CgInt), synthesised from the variable internally transcribed spacer (ITS) 1 region of ribosomal DNA (rDNA) of Collectotrichum gloeosporioides was used for PCR with primer ITS4 (from a conserved sequence of the rDNA) to amplify a 450-bp fragment from the 25 C. gloeosporioides isolates tested. This specific fragment was amplified from as little as 10 fg of fungal DNA. A similar sized fragment was amplified from DNA extracted from C. gloeosporioides-infected tomato tissue. RAPD analysis divided 39 C. gloeosporioides isolates into more than 12 groups linked to host source and geographic origin. Based on the results obtained, the potential of PCR for detection and differentiation of C. gloeosporioides is discussed.
从胶孢炭疽菌核糖体DNA（rDNA）的可变内部转录间隔区（ITS）1区域合成的寡核苷酸引物（CgInt），与引物ITS4（来自rDNA的保守序列）一起用于PCR，以从测试的25株胶孢炭疽菌分离物中扩增出一个450 bp的片段。这个特定片段可从低至10 fg的真菌DNA中扩增出来。从感染胶孢炭疽菌的番茄组织中提取的DNA也扩增出了类似大小的片段。RAPD分析将39株胶孢炭疽菌分离物分为12个以上与寄主来源和地理起源相关的组。根据所得结果，讨论了PCR在检测和区分胶孢炭疽菌方面的潜力。
