Morozzi G, Cenci G, Scardazza F, Pitzurra M
Microbios. 1986;48(194):27-35.
The present study evaluates the effect of the cadmium (Cd2+) on the growth and protein synthesis of some Gram-positive (Staphylococcus aureus, Bacillus subtilis and Streptococcus faecium) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria and the cadmium uptake by the same micro-organisms. The Gram-negative bacteria tested were less sensitive to metal ions than the Gram-positive, and P. aeruginosa was the most resistant. The Gram-negative bacteria were also able to accumulate higher amounts of cadmium during growth than the Gram-positive bacteria. The maximum values of specific metal uptake (microgram of Cd2+ incorporated per mg of protein) were: 0.52 for S. aureus, 0.65 for S. faecium, 0.79 for B. subtilis, 2.79 for E. coli and 24.15 for P. aeruginosa, respectively. The differences in the ability to accumulate metal found between Gram-negative and Gram-positive bacteria seems to account for different mechanisms of metal resistance.
本研究评估了镉（Cd2+）对一些革兰氏阳性菌（金黄色葡萄球菌、枯草芽孢杆菌和粪肠球菌）和革兰氏阴性菌（大肠杆菌和铜绿假单胞菌）生长及蛋白质合成的影响，以及相同微生物对镉的摄取情况。所测试的革兰氏阴性菌对金属离子的敏感性低于革兰氏阳性菌，其中铜绿假单胞菌的抗性最强。革兰氏阴性菌在生长过程中积累镉的量也高于革兰氏阳性菌。特定金属摄取的最大值（每毫克蛋白质结合的Cd2+微克数）分别为：金黄色葡萄球菌0.52、粪肠球菌0.65、枯草芽孢杆菌0.79、大肠杆菌2.79、铜绿假单胞菌24.15。革兰氏阴性菌和革兰氏阳性菌在积累金属能力上的差异似乎解释了不同的金属抗性机制。
