具有广泛潜在生物活性的新型海洋胞外多糖：抗氧化、抗炎、细胞毒性及抗阿尔茨海默病活性研究
Novel Exopolysaccharide from Marine with Broad Potential Biological Activities: Insights into Antioxidant, Anti-Inflammatory, Cytotoxicity, and Anti-Alzheimer Activity.
作者信息
Abdel-Wahab Basel A, F Abd El-Kareem Hanaa, Alzamami Ahmad, A Fahmy Cinderella, H Elesawy Basem, Mostafa Mahmoud Maged, Ghareeb Ahmed, El Askary Ahmad, H Abo Nahas Hebatallah, G M Attallah Nashwah, Altwaijry Najla, M Saied Essa
机构信息
Department of Medical Pharmacology, College of Medicine, Assiut University, Assiut 7111, Egypt.
Department of Pharmacology, College of Pharmacy, Najran University, P.O. Box 1988, Najran 55461, Saudi Arabia.
出版信息
Metabolites. 2022 Jul 31;12(8):715. doi: 10.3390/metabo12080715.
In the presented study, strain AG4 isolated from marine was identified based on morphological, physiological, phylogenetic characteristics and an examination of 16S rRNA sequences. Novel exopolysaccharide (EPSR4) was extracted and isolated from the strain as a major fraction of exopolysaccharide (EPS). The analysis of structural characterization indicated that EPSR4 is a -glycosidic sulphated heteropolysaccharide (48.2%) with a molecular weight (Mw) of 1.48 × 10 g/mole and has no uronic acid. Analysis of monosaccharide content revealed that EPSR4 consists of glucose, rhamnose and arabinose monosaccharide in a molar ratio of 5:1:3, respectively. Morphological analysis revealed that EPSR4 possess a high crystallinity degree with a significant degree of porosity, and its aggregation and conformation in the lipid phase might have a significant impact on the bioactivity of EPSR4. The biological activity of EPSR4 was screened and evaluated by investigating its antioxidant, cytotoxicity, anti-inflammatory, and anti-Alzheimer activities. The antioxidant activity results showed that EPSR4 has 97.6% scavenging activity toward DPPH free radicals at 1500 µg/mL, with an IC value of 300 µg/mL, and 64.8% at 1500 µg/mL toward hydrogen peroxide free radicals (IC = 1500 µg/mL, 30 min). Furthermore, EPSR4 exhibited considerable inhibitory activity towards the proliferation of T-24 (bladder carcinoma), A-549 (lung cancer) and HepG-2 (hepatocellular carcinoma) cancer cell lines with IC of 244 µg/mL, 148 µg/mL and 123 µg/mL, respectively. An evaluation of anti-inflammatory activity revealed that EPSR4 has potent lipoxygenase (LOX) inhibitory activity (IC of 54.3 µg/mL) and a considerable effect on membrane stabilization (IC = 112.2 ± 1.2 µg/mL), while it showed cyclooxygenase (COX2) inhibitory activity up to 125 µg/mL. Finally, EPSR4 showed considerable inhibitory activity towards acetylcholine esterase activity. Taken together, this study reveals that strain AG4 could be considered as a potential natural source of novel EPS with potent biological activities that would be useful for the healthcare system.
在本研究中，基于形态学、生理学、系统发育特征以及16S rRNA序列分析，对从海洋中分离出的AG4菌株进行了鉴定。从该菌株中提取并分离出了新型胞外多糖（EPSR4），作为胞外多糖（EPS）的主要成分。结构表征分析表明，EPSR4是一种α-糖苷硫酸化杂多糖（48.2%），分子量（Mw）为1.48×10 g/摩尔，且不含糖醛酸。单糖含量分析显示，EPSR4由葡萄糖、鼠李糖和阿拉伯糖单糖组成，摩尔比分别为5:1:3。形态学分析表明，EPSR4具有高结晶度和显著的孔隙率，其在脂质相中的聚集和构象可能对EPSR4的生物活性有显著影响。通过研究其抗氧化、细胞毒性、抗炎和抗阿尔茨海默病活性，对EPSR4的生物活性进行了筛选和评估。抗氧化活性结果表明，EPSR4在1500 µg/mL时对DPPH自由基的清除活性为97.6%，IC值为300 µg/mL，在1500 µg/mL时对过氧化氢自由基的清除活性为64.8%（IC = 1500 µg/mL，30分钟）。此外，EPSR4对T-24（膀胱癌）、A-549（肺癌）和HepG-2（肝癌）癌细胞系的增殖具有显著抑制活性，IC值分别为244 µg/mL、148 µg/mL和123 µg/mL。抗炎活性评估显示，EPSR4具有强大的脂氧合酶（LOX）抑制活性（IC值为54.3 µg/mL），对膜稳定有显著作用（IC = 112.2 ± 1.2 µg/mL），同时在高达125 µg/mL时显示出环氧合酶（COX2）抑制活性。最后，EPSR4对乙酰胆碱酯酶活性显示出显著抑制活性。综上所述，本研究表明，AG4菌株可被视为具有强大生物活性的新型EPS的潜在天然来源，这对医疗保健系统将是有用的。
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