弯曲杆菌属物种染色体DNA中GATC序列中甲基化腺嘌呤的存在。
Presence of methylated adenine in GATC sequences in chromosomal DNAs from Campylobacter species.
作者信息
Edmonds P, Hall B M, Edwards W R, Hartline K M
机构信息
School of Biology, Georgia Institute of Technology, Atlanta 30332.
出版信息
J Bacteriol. 1992 Dec;174(24):8156-7. doi: 10.1128/jb.174.24.8156-8157.1992.
We digested chromosomal DNAs from 12 Campylobacter strains (C. jejuni, 4 strains; C. coli, 2 strains; C. fetus subsp. fetus, 2 strains; C. hyointestinalis, 2 strains; and C. upsaliensis, 2 strains) and from 4 Helicobacter strains (H. pylori, 2 strains; and H. mustelae, 2 strains) with HindIII, SstI, BamHI, DpnI, MboI, and Sau3AI. Restriction fragments were then separated by electrophoresis in 1% agarose or 10% polyacrylamide gels. Only DNAs from three Campylobacter species (C. jejuni, C. coli, and C. upsaliensis) were digested with DpnI (an enzyme that recognizes only methylated adenine in GATC sequences). We used MboI and Sau3AI to confirm these findings.
我们用HindIII、SstI、BamHI、DpnI、MboI和Sau3AI对12株弯曲杆菌菌株（空肠弯曲杆菌4株；结肠弯曲杆菌2株；胎儿弯曲杆菌胎儿亚种2株；猪肠弯曲杆菌2株；乌普萨拉弯曲杆菌2株）以及4株幽门螺杆菌菌株（幽门螺杆菌2株；鼬源幽门螺杆菌2株）的染色体DNA进行了消化。然后，通过在1%琼脂糖凝胶或10%聚丙烯酰胺凝胶中进行电泳来分离限制性片段。只有来自三种弯曲杆菌（空肠弯曲杆菌、结肠弯曲杆菌和乌普萨拉弯曲杆菌）的DNA用DpnI（一种仅识别GATC序列中甲基化腺嘌呤的酶）进行了消化。我们使用MboI和Sau3AI来证实这些发现。
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