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火鸡和猪源弯曲杆菌基因组 DNA 中 GATC 位点甲基化的差异。

Differences in methylation at GATC sites in genomic DNA of Campylobacter coli from turkeys and swine.

机构信息

North Carolina State University, Department of Food Science, 339 Schaub Hall, Raleigh, NC 27695, USA.

出版信息

Appl Environ Microbiol. 2010 Nov;76(21):7314-7. doi: 10.1128/AEM.00934-10. Epub 2010 Sep 10.

Abstract

A significant fraction (46/108, 43%) of swine isolates of Campylobacter coli but none of 81 isolates of C. coli from turkeys had genomic DNA that was resistant to digestion by MboI, suggesting methylation of adenines at GATC sites. No consistent association was noted between antimicrobial resistance and MboI resistance. Seven swine-associated multilocus sequence typing-based sequence types (STs) were detected among multiple isolates with MboI-resistant DNA. The data suggest host-associated DNA modification system(s) specific for adenine at GATC sites in C. coli from swine.

摘要

猪源弯曲杆菌属空肠弯曲菌的很大一部分(46/108,43%)分离株但没有 81 株来自火鸡的弯曲杆菌属空肠弯曲菌分离株的基因组 DNA 对 MboI 的消化具有抗性,提示 GATC 位点腺嘌呤的甲基化。未发现抗生素耐药性与 MboI 耐药性之间存在一致的相关性。在具有 MboI 抗性 DNA 的多个分离株中检测到 7 种与猪相关的多位点序列分型(ST)。这些数据表明猪源弯曲杆菌属空肠弯曲菌中存在宿主相关的 GATC 位点腺嘌呤特异性 DNA 修饰系统。

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