Leonhardt U, Fayyazzi A, Seidensticker F, Stöckmann F, Söling H D, Creutzfeldt W
Department of Medicine, University of Göttingen, Germany.
Int J Pancreatol. 1992 Oct;12(2):161-6. doi: 10.1007/BF02924640.
This study investigates the role of platelet-activating factor (PAF) in experimental pancreatitis. The concentration of PAF quantified in ascites of bile-induced pancreatitis by radioimmunoassay (RIA) ranged from 3.67 +/- 0.39 pmol/mL 2 h to 0.954 +/- 0.39 pmol/mL 10 h after injection of taurocholate. Administration of a potent PAF antagonist, WEB-2170, prior to injection of taurocholate prolonged mean survival time in rats receiving i.v. camostate and albumin (46.4 h, n = 15, vs controls 38.3 h, n = 13). However, the survival rate after 72 h was not improved. The histologically estimated severity of pancreatitis and pancreatic enzymes in blood, tissue, or ascites was not affected. WEB-2170 had no effect on survival when injected simultaneously with taurocholate into the pancreatic duct or given i.v. after induction of pancreatitis (1, 0.1, or 0.01 mg/kg WEB-2170 vs controls). Subcutaneous injection of 10 mg/kg WEB-2170 also did not improve survival in pancreatitis induced by choline-deficient, ethionine-supplemented diet in mice. It is concluded that administration of a PAF antagonist after the onset of severe experimental pancreatitis does not influence its outcome, although activation of PAF may play a role in the pathogenesis of pancreatitis.
本研究探讨血小板活化因子（PAF）在实验性胰腺炎中的作用。通过放射免疫分析（RIA）定量测定胆盐诱导的胰腺炎腹水中PAF的浓度，在注射牛磺胆酸盐后2小时为3.67±0.39 pmol/mL，10小时为0.954±0.39 pmol/mL。在注射牛磺胆酸盐之前给予强效PAF拮抗剂WEB-2170，可延长接受静脉注射抑肽酶和白蛋白的大鼠的平均存活时间（46.4小时，n = 15，对照组为38.3小时，n = 13）。然而，72小时后的存活率并未提高。胰腺炎的组织学估计严重程度以及血液、组织或腹水中的胰腺酶均未受影响。当与牛磺胆酸盐同时注入胰管或在胰腺炎诱导后静脉注射（1、0.1或0.01 mg/kg WEB-2170与对照组相比）时，WEB-2170对存活没有影响。皮下注射10 mg/kg WEB-2170也不能提高胆碱缺乏、补充乙硫氨酸饮食诱导的小鼠胰腺炎的存活率。结论是，在严重实验性胰腺炎发作后给予PAF拮抗剂不会影响其结局，尽管PAF的激活可能在胰腺炎的发病机制中起作用。
