在有培养记录的生殖器疱疹病例中，检测单纯疱疹病毒2型糖蛋白G抗体的焦点法和卡隆酶联免疫吸附测定法的性能。
Performance of the focus and Kalon enzyme-linked immunosorbent assays for antibodies to herpes simplex virus type 2 glycoprotein G in culture-documented cases of genital herpes.
作者信息
Morrow Rhoda Ashley, Friedrich David, Krantz Elizabeth
机构信息
University of Washington Department of Laboratory Medicine, Children's Hospital and Regional Medical Center, Seattle, Washington 98195, USA.
出版信息
J Clin Microbiol. 2003 Nov;41(11):5212-4. doi: 10.1128/JCM.41.11.5212-5214.2003.
Abstract
Glycoprotein G-based herpes simplex virus type 2 (HSV-2) enzyme-linked immunosorbent assays from Focus and Kalon were performed with specimens from 118 patients with culture-documented genital herpes episodes, and their results were compared. Sensitivity was 52% by Kalon and 86% by Focus for first HSV-2 episodes and 100% (for each of the two tests) for recurrent HSV-2. Median times to seroconversion were 120 days by the Kalon assay, 21 days by the Focus assay, and 68 days by Western blotting assay. Values for specificity were 100% (Kalon) and 93% (Focus).
摘要
使用Focus和Kalon公司基于糖蛋白G的2型单纯疱疹病毒（HSV-2）酶联免疫吸附测定法，对118例有培养记录的生殖器疱疹发作患者的标本进行检测，并比较结果。对于首次HSV-2发作，Kalon法的敏感性为52%，Focus法为86%；对于复发性HSV-2，两种检测方法的敏感性均为100%。通过Kalon测定法血清转化的中位时间为120天，Focus测定法为21天，蛋白质印迹法为68天。Kalon法的特异性值为100%，Focus法为93%。
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