Liming Xia, Xueliang Shen
Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou 31002,7 PR China.
Bioresour Technol. 2004 Feb;91(3):259-62. doi: 10.1016/s0960-8524(03)00195-0.
Cellulase production using corn cob residue from xylose manufacture as substrate was carried out by Trichoderma reesei ZU-02. It was found that on the same cellulose basis, the cellulase activity and yield produced on corn cob residue were comparable with that on purified cellulose. Under batch process, the optimum concentration of substrate was 40 g/l and the optimum C/N ratio was 8.0. In 500 ml flasks, cellulase activity reached 5.25 IU/ml (213.4 IU/g cellulose) after seven days' cultivation. In a 30 m(3) stirred fermenter for large scale production, cellulase and cellobiase activity were 5.48 IU/ml (222.8 IU/g cellulase) and 0.25 IU/ml (10.2 IU/g cellulose), respectively, after four days' submerged fermentation. The produced cellulase could effectively hydrolyze the corn cob residue, and the yield of enzymatic hydrolysis reached 90.4% on 10% corn cob residue (w/v) when the cellulase dosage was 20 IU/g substrate.
里氏木霉ZU - 02以木糖生产过程中的玉米芯残渣为底物进行纤维素酶的生产。结果发现，在相同纤维素基础上，玉米芯残渣产生的纤维素酶活性和产量与纯化纤维素相当。在分批培养过程中，底物的最佳浓度为40 g/l，最佳碳氮比为8.0。在500 ml烧瓶中，培养7天后纤维素酶活性达到5.25 IU/ml（213.4 IU/g纤维素）。在30 m³搅拌发酵罐中进行大规模生产时，经过4天的深层发酵，纤维素酶和纤维二糖酶活性分别为5.48 IU/ml（222.8 IU/g纤维素酶）和0.25 IU/ml（10.2 IU/g纤维素）。所产生的纤维素酶能够有效水解玉米芯残渣，当纤维素酶用量为20 IU/g底物时，在10%（w/v）的玉米芯残渣上酶解产率达到90.4%。
