Enzymology Laboratory, University of Brasília, Campus Universitário Darcy Ribeiro, Brasília, DF, Brazil.
Laboratory of Chemistry, Cellulose and Energy, Department of Forest Sciences, ESALQ, University of São Paulo, Piracicaba, SP, Brazil.
Folia Microbiol (Praha). 2022 Oct;67(5):721-732. doi: 10.1007/s12223-022-00971-8. Epub 2022 Apr 22.
To better understand the production of enzymes of industrial interest from microorganisms with biotechnological potential using lignocellulosic biomass, we evaluated the production of endoglucanase and xylanase from Aspergillus tamarii. CAZymes domains were evaluated in the genome, and a screening of the enzymatic potential of A. tamarii in various agricultural biomasses was done. The enzymatic profile could be associated with the biomass complexity, with increased biomass recalcitrance yielding higher activity. A time-course profile defined 48 h of cultivation as the best period for cultivating A. tamarii in sugarcane bagasse reached 12.05 IU/mg for endoglucanase and 74.86 IU/mg for xylanase. Using 0.1% (w/v) tryptone as the only nitrogen source and 12 µmol/L CuSO addition had an overall positive effect on the enzymatic activity and protein production. A 2 factorial central composite design was used then to investigate the simultaneous influence of tryptone and CuSO on enzyme activity. Tryptone strongly affected enzymatic activity, decreasing endoglucanase activity but increasing xylanase activity. CuSO supplementation was advantageous for endoglucanases, increasing their activity, and it had a negative effect on xylanases. But overall, the experimental design increased the enzymatic activity of all biomasses used. For the clean cotton residue, the experimental design was able to reach the highest enzyme activity for endoglucanase and xylanase, with 1.195 IU/mL and 6.353 IU/mL, respectively. More experimental studies are required to investigate how the biomass induction effect impacts enzyme production.
为了更好地利用木质纤维素生物质生产具有生物技术潜力的微生物的工业感兴趣的酶，我们评估了 Aspergillus tamarii 生产内切葡聚糖酶和木聚糖酶的能力。在基因组中评估了 CAZymes 结构域，并对 A. tamarii 在各种农业生物量中的酶潜力进行了筛选。酶谱可以与生物质的复杂性相关联，随着生物质的顽固性增加，活性也会增加。时间过程曲线将培养 48 小时定义为在甘蔗渣中培养 A. tamarii 的最佳时间，内切葡聚糖酶和木聚糖酶的活性分别达到 12.05 IU/mg 和 74.86 IU/mg。使用 0.1%（w/v）的胰蛋白胨作为唯一的氮源和 12 μmol/L 的 CuSO 添加对酶活性和蛋白质生产有整体的积极影响。然后使用 2 因子中心复合设计来研究胰蛋白胨和 CuSO 对酶活性的同时影响。胰蛋白胨强烈影响酶活性，降低内切葡聚糖酶的活性，但增加木聚糖酶的活性。CuSO 的补充有利于内切葡聚糖酶，增加其活性，对木聚糖酶则有负面影响。但总的来说，实验设计提高了所有使用的生物质的酶活性。对于干净的棉绒残余物，实验设计能够达到内切葡聚糖酶和木聚糖酶的最高酶活，分别为 1.195 IU/mL 和 6.353 IU/mL。需要进行更多的实验研究来研究生物质诱导效应对酶生产的影响。
