Sen Chandan K
Dorothy M Davis Heart and Lung Research Institute, Department of Surgery, The Ohio State University Medical Center, Columbus, Ohio 43210, USA.
Wound Repair Regen. 2003 Nov-Dec;11(6):431-8. doi: 10.1046/j.1524-475x.2003.11607.x.
The orthodox view has been that reactive oxygen species are primarily damaging to cells. There is general agreement that while high (3%) doses of H(2)O(2) may serve as a clinical disinfectant, its overall effect on healing is not positive. Current work shows that at very low concentrations, reactive oxygen species may regulate cellular signaling pathways by redox-dependent mechanisms. Recent discoveries show that almost all cells of the wound microenvironment contain specialized enzymes that utilize O(2) to generate reactive oxygen species. Numerous aspects of wound healing are subject to redox control. An understanding of how endogenous reactive oxygen species are generated in wound-related cells may influence the healing process and could result in new redox-based therapeutic strategies. Current results with growth factor therapy of wounds have not met clinical expectations. Many of these growth factors, such as platelet-derived growth factor, rely on reactive oxygen species for functioning. Redox-based strategies may serve as effective adjuncts to jump-start healing of chronic wounds. The understanding of wound-site redox biology is also likely to provide novel insights into the fundamental mechanisms that would help to optimize conditions for oxygen therapy. While a window of therapeutic opportunity seems to exist under conditions of low concentrations of reactive oxygen species, high levels may complicate regeneration and remodeling of nascent tissue.
传统观点认为活性氧主要对细胞造成损害。人们普遍认为，虽然高剂量（3%）的过氧化氢可作为临床消毒剂，但其对伤口愈合的总体影响并不积极。目前的研究表明，在极低浓度下，活性氧可能通过氧化还原依赖机制调节细胞信号通路。最近的发现表明，伤口微环境中的几乎所有细胞都含有利用氧气生成活性氧的特殊酶。伤口愈合的许多方面都受氧化还原控制。了解伤口相关细胞中内源性活性氧的产生方式可能会影响愈合过程，并可能产生基于氧化还原的新治疗策略。目前伤口生长因子治疗的结果未达到临床预期。许多这些生长因子，如血小板衍生生长因子，依赖活性氧发挥作用。基于氧化还原的策略可能作为有效的辅助手段来启动慢性伤口的愈合。对伤口部位氧化还原生物学的理解也可能为有助于优化氧疗条件的基本机制提供新的见解。虽然在低浓度活性氧条件下似乎存在治疗机会窗口，但高浓度可能会使新生组织的再生和重塑复杂化。
