粗糙脉孢菌细胞质起始tRNA的核苷酸序列。
Nucleotide sequence of Neurospora crassa cytoplasmic initiator tRNA.
作者信息
Gillum A M, Hecker L I, Silberklang M, Schwartzbach S D, RajBhandary U L, Barnett W E
出版信息
Nucleic Acids Res. 1977 Dec;4(12):4109-31. doi: 10.1093/nar/4.12.4109.
Initiator methionine tRNA from the cytoplasm of Neurospora crassa has been purified and sequenced. The sequence is: pAGCUGCAUm1GGCGCAGCGGAAGCGCM22GCYGGGCUCAUt6AACCCGGAGm7GU (or D) - CACUCGAUCGm1AAACGAGUUGCAGCUACCAOH. Similar to initiator tRNAs from the cytoplasm of other eukaryotes, this tRNA also contains the sequence -AUCG- instead of the usual -TphiCG (or A)- found in loop IV of other tRNAs. The sequence of the N. crassa cytoplasmic initiator tRNA is quite different from that of the corresponding mitochondrial initiator tRNA. Comparison of the sequence of N. crassa cytoplasmic initiator tRNA to those of yeast, wheat germ and vertebrate cytoplasmic initiator tRNA indicates that the sequences of the two fungal tRNAs are no more similar to each other than they are to those of other initiator tRNAs.
来自粗糙脉孢菌细胞质的起始甲硫氨酸tRNA已被纯化并测序。序列为：pAGCUGCAUm1GGCGCAGCGGAAGCGCM22GCYGGGCUCAUt6AACCCGGAGm7GU（或D）-CACUCGAUCGm1AAACGAGUUGCAGCUACCAOH。与其他真核生物细胞质中的起始tRNA相似，该tRNA在环IV中也含有序列-AUCG-，而不是其他tRNA中常见的-TphiCG（或A）-。粗糙脉孢菌细胞质起始tRNA的序列与相应线粒体起始tRNA的序列有很大不同。将粗糙脉孢菌细胞质起始tRNA的序列与酵母、小麦胚芽和脊椎动物细胞质起始tRNA的序列进行比较表明，两种真菌tRNA的序列彼此之间的相似性并不高于它们与其他起始tRNA的相似性。
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