Arias Irigoyen J, Talavera Fabuel A, Maranon Lizana F
Department of Internal Medicine, Gastroenterology and Hepatology, Hospital Infanta Elena, Huelva, Spain.
J Investig Allergol Clin Immunol. 2003;13(3):213-5.
A 44-year-old subject developed rhinoconjunctivitis symptoms when she was exposed to white pepper while working in the food industry. A positive skin prick test for white and black pepper extracts (1:10 w/v) were obtained. Specific IgE antibodies to white and black pepper were demonstrated by ELISA. The immunoblot analysis showed two IgE-reactive protein bands able to bind to IgE from white pepper extract of 11.8 kDa and 13.6 kDa and one band from black pepper extract of 11.8 kDa. IgE binding to blotted white and black pepper extract were inhibited by preincubation of patient serum with black pepper extract. A conjunctival provocation test was positive with a white pepper extract dilution of 1:100 w/v. We describe a patient with occupational rhinoconjunctivitis caused by hypersensitivity to white pepper.
一名44岁的受试者在食品行业工作时接触白胡椒后出现了鼻结膜炎症状。对白胡椒和黑胡椒提取物（1:10 w/v）进行皮肤点刺试验呈阳性。通过酶联免疫吸附测定法检测到对白胡椒和黑胡椒的特异性IgE抗体。免疫印迹分析显示，白胡椒提取物中有两条能与IgE结合的反应性蛋白带，分子量分别为11.8 kDa和13.6 kDa，黑胡椒提取物中有一条11.8 kDa的带。患者血清与黑胡椒提取物预孵育可抑制IgE与印迹的白胡椒和黑胡椒提取物的结合。用1:100 w/v的白胡椒提取物稀释液进行结膜激发试验呈阳性。我们描述了一名因对白胡椒过敏而导致职业性鼻结膜炎的患者。
