Dekonenko Alexander, Yakimenko Valeriy, Ivanov Alexander, Morozov Vyacheslav, Nikitin Pavel, Khasanova Samara, Dzagurova Tamara, Tkachenko Evgeniy, Schmaljohn Connie
Chumakov Institute of Poliomyelitis and Viral Encephalitides RAMS, Moscow, Russia.
Infect Genet Evol. 2003 Nov;3(4):245-57. doi: 10.1016/s1567-1348(03)00088-1.
A total of 678 small mammals representing eight species were trapped in western Siberia in 1999-2000 and assayed for the presence of hantaviruses. Eighteen animals, all Clethrionomys species, were antigen positive by enzyme-linked immunosorbent assay (ELISA). Small and medium genome segments were recovered by RT-PCR from six samples from Clethrionomys glareolus and three from Clethrionomys rufocanus. Sequence comparison and phylogenetic analysis revealed that these hantaviruses were Puumala virus and were similar to hantavirus strains from Finland. To confirm these data, partial nucleotide sequences of the rodent hosts' cytochrome b genes were obtained, as well as several sequences from genes from rodents trapped at different localities of European Russia and western Siberia. The cytochrome b sequences of Siberian bank voles were similar to sequences of C. glareolus, trapped in Finland. These data suggest that the Puumala hantaviruses, as well as their rodent hosts, share a common evolutionary history. We propose that these rodents and viruses may be descendents of a population of bank voles that expanded northward from southern refugia during one of the interglacial periods.
1999年至2000年期间，在西西伯利亚共捕获了代表8个物种的678只小型哺乳动物，并检测了是否存在汉坦病毒。通过酶联免疫吸附测定（ELISA），18只动物（均为棕背䶄属物种）抗原呈阳性。通过逆转录聚合酶链反应（RT-PCR）从6份来自普通田鼠的样本和3份来自红背䶄的样本中回收了小基因组片段和中基因组片段。序列比较和系统发育分析表明，这些汉坦病毒是普马拉病毒，与来自芬兰的汉坦病毒株相似。为了证实这些数据，获得了啮齿动物宿主细胞色素b基因的部分核苷酸序列，以及从欧洲俄罗斯和西西伯利亚不同地点捕获的啮齿动物的几个基因序列。西伯利亚棕背田鼠的细胞色素b序列与在芬兰捕获的普通田鼠的序列相似。这些数据表明，普马拉汉坦病毒及其啮齿动物宿主有着共同的进化历史。我们认为，这些啮齿动物和病毒可能是在一次间冰期从南方避难所向北扩张的一群棕背田鼠的后代。
