Kabwe Emmanuel, Al Sheikh Walaa, Shamsutdinov Anton F, Ismagilova Ruzilya K, Martynova Ekaterina V, Ohlopkova Olesia V, Yurchenko Yuri A, Savitskaya Tatiana A, Isaeva Guzel S, Khaiboullina Svetlana F, Rizvanov Albert A, Morzunov Sergey P, Davidyuk Yuriy N
OpenLab "Gene and Cell Technologies ", Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia.
Kazan Research Institute of Epidemiology and Microbiology, 420012 Kazan, Russia.
Trop Med Infect Dis. 2022 Mar 6;7(3):46. doi: 10.3390/tropicalmed7030046.
Hemorrhagic fever with renal syndrome (HFRS) is a zoonotic disease commonly diagnosed in the Volga Federal District (VFD). HFRS is caused by Puumala orthohantavirus (PUUV), and this virus is usually detected in bank voles as its natural host (Myodes glareolus). The PUUV genome is composed of the single-stranded, negative-sense RNA containing three segments. The goal of the current study is to identify genome variants of PUUV strains circulating in bank voles captured in the Udmurt Republic (UR) and Ulyanovsk region (ULR). The comparative and phylogenetic analysis of PUUV strains revealed that strains from Varaksino site UR are closely related to strains previously identified in the Pre-Kama area of the Republic of Tatarstan (RT), whilst strains from Kurlan and Mullovka sites ULR are similar to strains from the Trans-Kama area of the RT. It was also found that Barysh ULR strains form a separate distinct group phylogenetically equidistant from Varaksino and Kurlan−Mullovka groups. The identified groups of strains can be considered as separate sub-lineages in the PUUV Russian genetic lineage. In addition, the genomes of the strains from the UR, most likely, were formed as a result of reassortment.
肾综合征出血热（HFRS）是一种人畜共患病，在伏尔加联邦区（VFD）较为常见。HFRS由普马拉正汉坦病毒（PUUV）引起，该病毒通常在作为其自然宿主的棕背鼠平（Myodes glareolus）中被检测到。PUUV基因组由包含三个片段的单链负义RNA组成。本研究的目的是鉴定在乌德穆尔特共和国（UR）和乌里扬诺夫斯克地区（ULR）捕获的棕背鼠平中传播的PUUV毒株的基因组变异。对PUUV毒株的比较和系统发育分析表明，来自UR的瓦拉西诺地点的毒株与先前在鞑靼斯坦共和国（RT）卡马河上游地区鉴定的毒株密切相关，而来自ULR的库尔兰和穆洛夫卡地点的毒株与RT跨卡马地区的毒株相似。还发现，ULR的巴里什毒株在系统发育上形成了一个独立的独特群体，与瓦拉西诺和库尔兰 - 穆洛夫卡群体等距。所鉴定的毒株群体可被视为PUUV俄罗斯遗传谱系中的独立亚谱系。此外，UR毒株的基因组很可能是重组的结果。
