Yuasa Tetsuya, Miyamoto Youji, Ishikawa Kunio, Takechi Masaaki, Momota Yukihiro, Tatehara Seiko, Nagayama Masaru
First Department of Oral and Maxillofacial Surgery, School of Dentistry, Tokushima University, 3-18-15 Kuramoto, Tokushima 770-8504, Japan.
Biomaterials. 2004 Mar-Apr;25(7-8):1159-66. doi: 10.1016/j.biomaterials.2003.08.003.
Although apatite cement (AC) and sintered hydroxyapatite (s-HAP) are known to show good osteoconductivity, it is not clear whether or not the degree of their osteoconductivity is the same. In addition, it has not been clarified whether or not it is dependent on the type of AC; conventional AC (c-AC), fast-setting AC (fs-AC) or anti-washout AC (aw-AC). The aim of this study was to investigate the effects of ACs on cultured human osteoblasts, as they may provide a useful index of osteoconductivity. Human osteoblasts were cultured on the surfaces of ACs and s-HAP, and were evaluated with respect to cell attachment, proliferation, and differentiation. We found that ACs and s-HAP showed similar cell attachment. No significant difference between ACs and s-HAP was found with respect to the proliferation of osteoblasts. In contrast, we found that the differentiation of osteoblasts was enhanced on the surface of ACs compared with that of s-HAP. However, there was no difference among the types of AC. We therefore concluded that AC may show better osteoconductivity than s-HAP, and that osteoconductivity of AC may be similar, regardless of the type of AC.
尽管磷灰石骨水泥（AC）和烧结羟基磷灰石（s-HAP）已知具有良好的骨传导性，但尚不清楚它们的骨传导程度是否相同。此外，也未阐明其是否取决于AC的类型；传统AC（c-AC）、速凝AC（fs-AC）还是抗冲洗AC（aw-AC）。本研究的目的是研究AC对培养的人成骨细胞的影响，因为它们可能提供骨传导性的有用指标。将人成骨细胞培养在AC和s-HAP的表面，并对细胞附着、增殖和分化进行评估。我们发现AC和s-HAP表现出相似的细胞附着。在成骨细胞增殖方面，AC和s-HAP之间未发现显著差异。相比之下，我们发现与s-HAP相比，AC表面的成骨细胞分化增强。然而，不同类型的AC之间没有差异。因此，我们得出结论，AC可能比s-HAP表现出更好的骨传导性，并且无论AC的类型如何，其骨传导性可能相似。
