Sasaki Kazuki, Ninomiya Yoshiaki, Takechi Masaaki, Tsuru Kanji, Ishikawa Kunio, Shigeishi Hideo, Ohta Kouji, Aikawa Tomonao
Department of Oral and Maxillofacial Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
Department of Dentistry, Oral and Maxillofacial Surgery, National Hospital Organization Kure Medical Center and Chugoku Cancer Center, Kure 737-0023, Japan.
Materials (Basel). 2023 Jan 21;16(3):995. doi: 10.3390/ma16030995.
Apatite cement (AC), which has excellent osteoconductive ability, and alpha-tricalcium phosphate (α-TCP), which can be used for bone replacement, are useful bone substitute materials. The objective of this study was to clarify the physical properties and antimicrobial release ability of antibiotic-loaded AC/α-TCP composites in vitro. Gentamicin-loaded, rapid setting AC/α-TCP composites were prepared in 2 mixing ratios (10:3 and 10:6). The cement paste of AC/α-TCP composites was prepared in a plastic mold and dried in a thermostatic chamber at 37 °C and 100% relative humidity for 24 h. A diametral tensile strength test, powder X-ray diffraction analysis, and gentamicin release test were performed. The diametral tensile strengths of the AC/α-TCP composites were significantly less than that of AC alone. Powder X-ray diffraction patterns exhibited the characteristic peaks of hydroxyapatite in the AC/α-TCP composites and gentamicin-loaded AC/α-TCP composites. The concentration of the released gentamicin was maintained above the minimum inhibitory concentration of until Day 30 in both the gentamicin-loaded AC/α-TCP composites (10:3 and 10:6). Our results suggest that a gentamicin-loaded AC/α-TCP composite has potential as a drug delivery system. Further study is essential to investigate the antimicrobial activity and safety of the gentamicin-loaded AC/α-TCP composites in animal models.
具有优异骨传导能力的磷灰石水泥（AC）和可用于骨替代的α-磷酸三钙（α-TCP）是有用的骨替代材料。本研究的目的是阐明载抗生素AC/α-TCP复合材料的物理性能和体外抗菌释放能力。以两种混合比例（10:3和10:6）制备了载庆大霉素的速凝AC/α-TCP复合材料。AC/α-TCP复合材料的水泥浆在塑料模具中制备，并在37℃、相对湿度100%的恒温箱中干燥24小时。进行了径向拉伸强度测试、粉末X射线衍射分析和庆大霉素释放测试。AC/α-TCP复合材料的径向拉伸强度明显低于单独的AC。粉末X射线衍射图谱显示了AC/α-TCP复合材料和载庆大霉素的AC/α-TCP复合材料中羟基磷灰石的特征峰。在载庆大霉素的AC/α-TCP复合材料（10:3和10:6）中，释放的庆大霉素浓度在第30天之前一直保持在最低抑菌浓度以上。我们的结果表明，载庆大霉素的AC/α-TCP复合材料具有作为药物递送系统的潜力。进一步研究载庆大霉素的AC/α-TCP复合材料在动物模型中的抗菌活性和安全性至关重要。
