Capo-Chichi L J A, Morton C M, Weaver D B
Department of Agronomy and Soils, Auburn University, 202 Funchess Hall, AL 36849-5412, USA,
Theor Appl Genet. 2004 Mar;108(5):814-21. doi: 10.1007/s00122-003-1493-8. Epub 2003 Nov 29.
Velvetbean ( Mucuna sp., n=11), a self-pollinated species, is an important legume used in tropical agricultural systems in rotation with other crops for nematode management and/or soil improvement. A genetic map of velvetbean was constructed in order to identify potential molecular markers linked to important morphological and agronomic traits that would be particularly useful for developing and improving the species. Traits such as seed coat color, pod color, and pod pubescence were among the main parameters observed in a process of genetic diversity estimation. Two slightly divergent velvetbean accessions, PI364362 and 'Edgar Farm White', a land race from Alabama, were used to make an intraspecific F(1) hybrid. Amplified fragment length polymorphism analysis (AFLP) detected an average of six polymorphic fragments per primer pair between the two parents. As expected for dominant markers, the sum of all AFLP bands from both parents was generally observed to be present in the AFLP profiles of the F(1) progeny, indicating full penetrance and the dominant nature of AFLP markers. An F(2) population was generated by self-pollinating a single F(1) plant. Using 37 AFLP primer pairs, we detected 233 polymorphic markers of which 164 (70.4%) segregated in 3:1 Mendelian ratios, while the remaining 69 (29.6%) both segregated and were scorable. The genetic linkage map constructed from this population comprised 166 markers, including two morphological traits (pod color and pod pubescence). Twenty linkage groups were found with an average distance between markers of 34.4 cM, covering a total of 687.9 cM. The linkage groups contained from 2 to 12 loci each and the distance between two consecutive loci ranged from 0 to 21.8 cM. The newly designated morphological traits pod color ( pdc) and pod pubescence ( pdp) co-segregated with each other at a distance of 4.2 cM. Two DNA markers designated ACGCAG2 and ACTCTG1 were located in the same group as pdc and pdp. The AFLP linkage map provides opportunities for use in marker-assisted selection and in the detection of loci controlling morphologically important traits.
黧豆（Mucuna sp.，n = 11）是一种自花授粉物种，是热带农业系统中一种重要的豆科植物，与其他作物轮作用于线虫管理和/或土壤改良。构建了黧豆的遗传图谱，以鉴定与重要形态和农艺性状相关的潜在分子标记，这对于该物种的培育和改良尤为有用。种皮颜色、豆荚颜色和豆荚茸毛等性状是遗传多样性评估过程中观察的主要参数。选用了两个略有差异的黧豆种质PI364362和来自阿拉巴马州的地方品种“Edgar Farm White”来进行种内F(1)杂交。扩增片段长度多态性分析（AFLP）检测到两个亲本之间每个引物对平均有6个多态性片段。正如显性标记所预期的那样，在F(1)后代的AFLP图谱中通常观察到来自两个亲本的所有AFLP条带的总和，这表明AFLP标记具有完全显性和显性性质。通过对单株F(1)植株进行自花授粉产生了一个F(2)群体。使用37对AFLP引物对，我们检测到233个多态性标记，其中164个（70.4%）以3:1的孟德尔比例分离，而其余69个（29.6%）既分离又可计分。从该群体构建的遗传连锁图谱包含166个标记，包括两个形态性状（豆荚颜色和豆荚茸毛）。发现了20个连锁群，标记之间的平均距离为34.4 cM，总共覆盖687.9 cM。每个连锁群包含2至12个位点，两个连续位点之间的距离范围为0至21.8 cM。新指定的形态性状豆荚颜色（pdc）和豆荚茸毛（pdp）在4.2 cM的距离处共分离。两个指定的DNA标记ACGCAG2和ACTCTG1与pdc和pdp位于同一组中。AFLP连锁图谱为标记辅助选择以及检测控制形态重要性状的基因座提供了机会。
