Yasui Yasuo, Wang Yingjie, Ohnishi Ohmi, Campbell Clayton G
Laboratory of Crop Evolution, Graduate School of Agriculture, Kyoto University, Muko, Japan.
Genome. 2004 Apr;47(2):345-51. doi: 10.1139/g03-126.
Common buckwheat (Fagopyrum esculentum) (2n = 2x = 16) and Fagopyrum homotropicum (2n = 2x = 16) were mated in an interspecific cross and amplified fragment length polymorphism (AFLP) linkage maps were constructed by analyzing segregation in the F2 population. Six hundred and sixty-nine bands were identified using 20 AFLP primer combinations, of which 462 (69%) segregated in the F2 population. The map of F. esculentum has eight linkage groups with 223 markers covering a total of 508.3 cM. The map of F. homotropicum has eight linkage groups with 211 markers covering 548.9 cM. There was one to one correspondence of the esculentum and homotropicum linkage groups. Three morphological markers, distylous self-incompatibility, shattering habit, and winged seed, were located on the AFLP map. Distylous self-incompatibility and shattering habit are tightly linked to each other (1.3 cM) and are located near the center of linkage group 1. Winged seed is located on linkage group 4.
普通荞麦（苦荞麦）（2n = 2x = 16）和同型荞麦（2n = 2x = 16）进行种间杂交，并通过分析F2群体的分离情况构建了扩增片段长度多态性（AFLP）连锁图谱。使用20对AFLP引物组合鉴定出669条带，其中462条（69%）在F2群体中分离。苦荞麦的图谱有8个连锁群，包含223个标记，覆盖总长度508.3厘摩。同型荞麦的图谱有8个连锁群，包含211个标记，覆盖548.9厘摩。苦荞麦和同型荞麦的连锁群存在一一对应关系。三个形态学标记，即二型花柱自交不亲和性、落粒习性和有翅种子，定位在AFLP图谱上。二型花柱自交不亲和性和落粒习性紧密连锁（1.3厘摩），位于连锁群1的中心附近。有翅种子位于连锁群4上。
