Bányai K, Jakab F, Reuter G, Bene J, Uj M, Melegh B, Szücs G
Regional Laboratory of Virology, Baranya County Institute of State Public Health Service, Szabadság út. 7, H-7621 Pécs, Hungary.
Arch Virol. 2003 Dec;148(12):2281-91. doi: 10.1007/s00705-003-0200-z. Epub 2003 Sep 19.
Human picobirnaviruses characterised in this study were serendipitously detected in a non-bacterial gastroenteritis outbreak when specimens were examined for the presence of human rotaviruses using polyacrylamide gel electrophoresis. Of ten stool samples sent for virological examination, two, three, and one specimens were positive for human caliciviruses, picobirnaviruses, and both viruses, respectively. Partial sequences of the RNA-dependent RNA polymerase gene were determined for three picobirnavirus-positive samples. The sequence identity among these three strains was 60% to 65% for the nucleic acid and 64% to 70% for the deduced amino acid sequences. Phylogenetic analysis revealed that each of the three strains clustered with strains identified in geographically separate areas. In contrast, human calicivirus strains co-incidentally identified, showed complete nucleotide sequence identity. These findings demonstrate a lack of common exposure to or point of source for picobirnavirus infection, suggesting that the outbreak was caused by human caliciviruses. Further studies are needed to determine the etiologic role and to establish the taxonomic basis of picobirnaviruses.
在本研究中鉴定的人微小双节段RNA病毒，是在一次非细菌性肠胃炎暴发期间意外检测到的。当时，使用聚丙烯酰胺凝胶电泳对标本进行检测，以确定是否存在人轮状病毒。在送去进行病毒学检查的10份粪便样本中，分别有2份、3份和1份样本对人杯状病毒、微小双节段RNA病毒以及这两种病毒均呈阳性。对3份微小双节段RNA病毒阳性样本测定了RNA依赖的RNA聚合酶基因的部分序列。这三个毒株之间的核酸序列同一性为60%至65%，推导的氨基酸序列同一性为64%至70%。系统发育分析表明，这三个毒株中的每一个都与在地理上不同区域鉴定出的毒株聚类。相比之下，偶然鉴定出的人杯状病毒毒株显示出完全相同的核苷酸序列。这些发现表明，微小双节段RNA病毒感染缺乏共同的暴露源或源头，提示该暴发是由人杯状病毒引起的。需要进一步研究以确定微小双节段RNA病毒的病因学作用并建立其分类学基础。
