太平洋莱茵海默氏菌新种，一种从太平洋深海海水中分离出的新型耐盐细菌。
Rheinheimera pacifica sp. nov., a novel halotolerant bacterium isolated from deep sea water of the Pacific.
作者信息
Romanenko Lyudmila A, Uchino Masataka, Falsen Enevold, Zhukova Natalia V, Mikhailov Valery V, Uchimura Tai
机构信息
Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, 690022 Vladivostok, Prospect 100 Let Vladivostoku, 159, Russia.
出版信息
Int J Syst Evol Microbiol. 2003 Nov;53(Pt 6):1973-7. doi: 10.1099/ijs.0.02252-0.
An aerobic, Gram-negative, non-fermentative, rod-shaped, motile, non-pigmented bacterium, KMM 1406(T), was isolated from a sample of Pacific deep sea water and investigated for phenotypic characteristics, chemotaxonomic features and phylogenetic relationships. The deep-sea isolate exhibited growth in 0-8 % (w/v) NaCl and at 4-37 degrees C, hydrolytic activity on gelatin, Tween 80 and starch and lack of D-glucose utilization. The major fatty acids were C(16 : 0), C(16 : 1)omega9c, C(17 : 1)omega8c and C(18 : 1)omega7c. The DNA G+C content was 49.6 mol%. 16S rRNA gene sequence analysis revealed that strain KMM 1406(T) was related closely to Rheinheimera baltica DSM 14885(T) within the gamma-Proteobacteria, with 96.8 % sequence similarity. On the basis of phenotypic and molecular data, a novel species, Rheinheimera pacifica sp. nov., is proposed. The type strain is KMM 1406(T) (=IAM 15043(T)=JCM 12090(T)=NRIC 0539(T)=CCUG 46544(T)).
从太平洋深海海水样本中分离出一株需氧、革兰氏阴性、非发酵、杆状、具运动性、无色素的细菌KMM 1406(T)，并对其进行了表型特征、化学分类特征和系统发育关系研究。该深海分离株在0-8%（w/v）NaCl浓度和4-37℃条件下生长，对明胶、吐温80和淀粉具有水解活性，且不能利用D-葡萄糖。主要脂肪酸为C(16 : 0)、C(16 : 1)ω9c、C(17 : 1)ω8c和C(18 : 1)ω7c。DNA G+C含量为49.6 mol%。16S rRNA基因序列分析表明，菌株KMM 1406(T)与γ-变形菌纲中的波罗的海莱茵海默氏菌DSM 14885(T)密切相关，序列相似性为96.8%。基于表型和分子数据，提出了一个新物种——太平洋莱茵海默氏菌（Rheinheimera pacifica sp. nov.）。模式菌株为KMM 1406(T)（=IAM 15043(T)=JCM 12090(T)=NRIC 0539(T)=CCUG 46544(T)）。
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