Hofmann Clemens, Aartsma Thijs J, Michel Hartmut, Köhler Jürgen
Experimental Physics IV and Bayreuther Institut für Makromolekülforschung, University of Bayreuth, 95440 Bayreuth, Germany.
Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15534-8. doi: 10.1073/pnas.2533896100. Epub 2003 Dec 11.
Single-molecule spectroscopic techniques were applied to individual pigments embedded in a chromoprotein. A sensitive tool to monitor structural fluctuations of the protein backbone in the local environment of the chromophore is provided by recording the changes of the spectral positions of the pigment absorptions as a function of time. The data provide information about the organization of the energy landscape of the protein in tiers that can be characterized by an average barrier height. Additionally, a correlation between the average barrier height within a distinct tier and the time scale of the structural fluctuations is observed.
单分子光谱技术被应用于嵌入色蛋白中的单个色素。通过记录色素吸收光谱位置随时间的变化,提供了一种监测发色团局部环境中蛋白质主链结构波动的灵敏工具。这些数据提供了有关蛋白质能量景观分层组织的信息,这些分层可以用平均势垒高度来表征。此外,还观察到不同分层内的平均势垒高度与结构波动时间尺度之间的相关性。