Chaveerach P, Lipman L J A, van Knapen F
Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80 175, 3508 TD Utrecht, The Netherlands.
Int J Food Microbiol. 2004 Jan 1;90(1):43-50. doi: 10.1016/s0168-1605(03)00170-3.
Chicken meat contaminated with Campylobacter jejuni can be the source of human enteritis. To decrease the risk of human infection, Campylobacter should be controlled at farm levels. Orally given probiotic bacteria could prevent colonisation of chicken with pathogenic bacteria like Campylobacter. The aim of this study was to investigate the effect of different bacteria on Campylobacter growth. Our results demonstrated that bacteria isolated from conventional chicken had potential inhibitory activities against Campylobacter. Other bacteria not isolated from chickens but with known antagonistic capacities, e.g. Enterococcus (56 strains) and Escherichia coli (20 strains), did not show any negative effect on Campylobacter. Interestingly, one Lactobacillus (P93) strain isolated from the chicken gut showed bactericidal activity against all tested Campylobacter. The bactericidal effect was characterised as the production of organic acids in combination with probably production of an anti-Campylobacter protein. In a co-culture study of Campylobacter and Lactobacillus (P93), the culturability of Campylobacter was under the detection limit after 48 h of incubation. A chicken experiment is needed to further evaluate the effect of the promising probiotic bacteria against Campylobacter colonisation in chicken.
被空肠弯曲菌污染的鸡肉可能是人类肠炎的源头。为降低人类感染风险，应在农场层面控制弯曲菌。口服益生菌可防止鸡肉被诸如弯曲菌等病原菌定植。本研究的目的是调查不同细菌对弯曲菌生长的影响。我们的结果表明，从传统养殖鸡中分离出的细菌对弯曲菌具有潜在抑制活性。其他未从鸡中分离出但具有已知拮抗能力的细菌，如肠球菌（56株）和大肠杆菌（20株），对弯曲菌未显示出任何负面影响。有趣的是，从鸡肠道分离出的一株乳酸杆菌（P93）对所有测试的弯曲菌均表现出杀菌活性。这种杀菌作用的特征是产生有机酸，可能还产生一种抗弯曲菌蛋白。在弯曲菌与乳酸杆菌（P93）的共培养研究中，孵育48小时后弯曲菌的可培养性低于检测限。需要进行一项鸡实验，以进一步评估这种有前景的益生菌对鸡中弯曲菌定植的影响。
