Moon H-S, Park W I, Choi E-A, Chung H-W, Kim S-C
Departments of Obstetrics and Gynecology, Ewha Womans University, College of Medicine, Seoul, Korea.
Int J Gynecol Cancer. 2003 Sep-Oct;13(5):640-6. doi: 10.1046/j.1525-1438.2003.13396.x.
The present study aimed to confirm the hypothesis that the expression and phosphorylation status of the E-cadherin/catenin adhesion complex is related to cervical carcinogenesis and cervical cancer invasion, and to investigate the expression and the tyrosine phosphorylation of focal adhesion kinase (FAK) and its relation with E-cadherin/catenin adhesion complex. The expression of E-cadherin, alpha- and beta-catenin, and FAK were studied by a western blot analysis with 26 cervical carcinomas, nine normal cervices, and five carcinomas in situ of cervix. The tyrosine phosphorylation of alpha- and beta-catenin and FAK were examined by an immunoprecipitation. The expressions of alpha- and beta-catenin and E-cadherin were reduced in cervical carcinoma, and the tyrosine phosphorylation of alpha- and beta-catenin in cervical carcinoma was higher than in normal cervix and carcinoma in situ of cervix. Tyrosine phosphorylation of FAK was elevated in cervical carcinoma although the expression of FAK was not significantly different. Moreover, alpha- and beta-catenin were coimmunoprecipitated with FAK. We conclude that the loss of E-cadherin/catenin proteins and the tyrosine phosphorylation of E-cadherin/catenin are involved in cervical carcinogenesis and cancer invasion. Tyrosine phosphorylation of focal adhesion kinase is also related to the cervical cancer invasion. The E-cadherin/catenin complex and FAK may be related functionally and structurally.
E-钙黏蛋白/连环蛋白黏附复合体的表达及磷酸化状态与宫颈癌发生及宫颈癌侵袭相关，并研究黏着斑激酶（FAK）的表达及其酪氨酸磷酸化情况，以及其与E-钙黏蛋白/连环蛋白黏附复合体的关系。采用蛋白质印迹分析法，对26例宫颈癌、9例正常宫颈组织及5例宫颈原位癌进行研究，检测E-钙黏蛋白、α-连环蛋白和β-连环蛋白以及FAK的表达。通过免疫沉淀法检测α-连环蛋白、β-连环蛋白和FAK的酪氨酸磷酸化情况。结果显示，宫颈癌组织中α-连环蛋白、β-连环蛋白和E-钙黏蛋白的表达降低，宫颈癌组织中α-连环蛋白和β-连环蛋白的酪氨酸磷酸化水平高于正常宫颈组织和宫颈原位癌。尽管FAK的表达无显著差异，但宫颈癌组织中FAK的酪氨酸磷酸化水平升高。此外，α-连环蛋白和β-连环蛋白与FAK可进行共免疫沉淀。我们得出结论，E-钙黏蛋白/连环蛋白的缺失及E-钙黏蛋白/连环蛋白的酪氨酸磷酸化参与宫颈癌的发生及侵袭过程。黏着斑激酶的酪氨酸磷酸化也与宫颈癌侵袭相关。E-钙黏蛋白/连环蛋白复合体与FAK可能在功能和结构上存在关联。
