Yin Hong, Zhang Dao-Chuan, Bi Zhi-Li, Yin Zhan, Liu Yong, Yin Xiang-Chu
College of Life Science, Hebei University, Baoding 071002, China.
Yi Chuan Xue Bao. 2003 Aug;30(8):766-72.
Based on the 16S rDNA of 8 species (belonging to 8 families of Acridoidea) from China and keyed to 8 relative species of Orthoptera from GenBank, the homologus sequences were compared. The used frequency of nucleotide was calculated and the molecular phylogenetic tree constructed by MEGA microsoft. In the 480 bp fragment of mitochondial 16S rDNA, A + T was about 70.7% and G + C only 29.3%. The sequence data revealed considerable variation in 188 nucleotide sites among the analyzed individuals from 8 different families. The variation rate of transversion was larger than or approach to transition. The results showed that the ranges of the 16S rDNA nucleotide divergence within species, among species of a genus, among genera of a family, and among the same family and suborder were 1.5%, 3.5%-3.6%, 4.8%-15.8% and 15.2%-25.6%, respectively. The members of the same genus and family were grouped together. The phylogenetic relationships of 8 families were Pyrgomorphidae-->Chrotogonidae-->Pamphagidae-->Oedipodidae-->Acrididae--> Arcypteridae-->Gomphoceridae-->Catantopidae. Pyrgomorphidae had closer relationships with Chrotogonidae as well as being the most ancestral group. Arcypteridae and Gomphoceridae were the sister groups and had closer relationships with most evolutionary Catantopidae. Tridactylidae was divided firstly as a suborder, which differs from the recent taxonomy distinctly. Haglidae (Tettigonoidea) and Gryllidae were grouped into suborder Ensifera, Tetrigidae and 8 families of Acridoidea were grouped into suborder Caelifera, as same as recent taxonomy.
基于中国8种蝗总科（隶属于8个科）昆虫的16S rDNA，并与GenBank中直翅目8个近缘物种进行比对，对同源序列进行了比较。计算了核苷酸的使用频率，并利用MEGA软件构建了分子系统发育树。在线粒体16S rDNA的480 bp片段中，A+T约占70.7%，G+C仅占29.3%。序列数据显示，来自8个不同科的分析个体在188个核苷酸位点上存在显著差异。颠换的变异率大于或接近转换。结果表明，16S rDNA核苷酸分歧在种内、属内种间、科内属间以及同科同亚目间的范围分别为1.5%、3.5%-3.6%、4.8%-15.8%和15.2%-25.6%。同属和同科的成员聚在一起。8个科的系统发育关系为：癞蝗科→瘤锥蝗科→斑翅蝗科→斑腿蝗科→蝗科→网翅蝗科→槌角蝗科→斑角蝗科。癞蝗科与瘤锥蝗科关系较近，且是最原始的类群。网翅蝗科和槌角蝗科是姐妹群，与最进化的斑角蝗科关系较近。蚤蝼科首先被划分为一个亚目，这与目前的分类明显不同。蛩蠊科（蛩蠊总科）和蟋蟀科被归入直翅亚目，蜢科和8个蝗总科的科被归入蝗亚目，与目前的分类相同。
