转化生长因子-β对兔颞下颌关节滑膜成纤维细胞中透明质酸合成代谢的影响

Effects of TGF-beta on hyaluronan anabolism in fibroblasts derived from the synovial membrane of the rabbit temporomandibular joint.

作者信息

Tanimoto K, Suzuki A, Ohno S, Honda K, Tanaka N, Doi T, Yoneno K, Ohno-Nakahara M, Nakatani Y, Ueki M, Tanne K

机构信息

Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.

出版信息

J Dent Res. 2004 Jan;83(1):40-4. doi: 10.1177/154405910408300108.

DOI:10.1177/154405910408300108

PMID:14691111

Abstract

Hyaluronan (HA) synthesis in the synovial membrane is affected by various chemical mediators. It is hypothesized that transforming growth factor-beta 1 (TGF-beta 1) would be a mediator to modulate HA synthesis in cultured synovial membrane fibroblasts of the temporomandibular joint (TMJ). Fibroblasts were extracted from the TMJ synovial membrane of four-week-old Japanese white rabbits. The amount of HA and expression levels of HA synthase (HAS) mRNAs induced by TGF-beta 1 treatment were analyzed by means of high-performance liquid chromatography and real-time polymerase chain-reaction, respectively. Both medium and large amounts of HA were enhanced by the stimulation of TGF-beta 1. HAS2 mRNA expression was enhanced 13-fold after six-hour stimulation with TGF-beta 1 (10 ng/mL), whereas HAS3 mRNA expression was not changed significantly. These results suggest that TGF-beta 1 enhances the expression of HAS2 mRNA in the TMJ synovial membrane fibroblasts and may contribute to the production of high-molecular-weight HA in the joint fluid.

摘要

滑膜中透明质酸（HA）的合成受多种化学介质影响。据推测，转化生长因子-β1（TGF-β1）可能是调节颞下颌关节（TMJ）滑膜成纤维细胞中HA合成的介质。从四周龄日本白兔的TMJ滑膜中提取成纤维细胞。分别通过高效液相色谱法和实时聚合酶链反应分析TGF-β1处理诱导的HA量和HA合酶（HAS）mRNA的表达水平。TGF-β1刺激可增强中量和大量HA的产生。用TGF-β1（10 ng/mL）刺激6小时后，HAS2 mRNA表达增强了13倍，而HAS3 mRNA表达无明显变化。这些结果表明，TGF-β1增强了TMJ滑膜成纤维细胞中HAS2 mRNA的表达，并可能有助于关节液中高分子量HA的产生。