Cochrane Lindsay, Ainscough Magdalen, Alfirevic Zarko
Liverpool Women's Hospital, Liverpool, UK.
Prenat Diagn. 2003 Dec 30;23(13):1049-51. doi: 10.1002/pd.752.
To determine the effect of needle and syringe size on the amount of tissue obtained at chorionic villus sampling
Two needle sizes, 18 and 20 gauge, and two syringe sizes 5 mL and 20 mL, were used to assess samples from term post-partum placentae. Each of the four combinations was tested by 25 aspirations. The placentae were divided into 100 grid spaces and each grid space was randomly allocated to a needle/syringe combination. The resulting samples were cleaned to separate the chorionic villi (CV), centrifuged and then weighed.
Significantly more tissue was obtained with an 18-g needle compared with a smaller 20-g needle (median weight difference 1.5 mg, 95% CI 0.8-2.3 mg). More tissue was also obtained with the larger 20-mL syringe though the impact of the syringe size was less than that of the needle size (median difference 0.8 mg, 95% CI 0-1.6).
A larger syringe and needle size yields a larger quantity of chorionic villi from the post-partum term placenta.
确定针头和注射器尺寸对绒毛取样时所获组织量的影响
使用两种针头尺寸（18号和20号）以及两种注射器尺寸（5毫升和20毫升）对足月产后胎盘样本进行评估。四种组合中的每一种均通过25次抽吸进行测试。将胎盘划分为100个网格区域，每个网格区域随机分配给一种针头/注射器组合。对所得样本进行清理以分离绒毛膜绒毛（CV），离心后称重。
与较细的20号针头相比，18号针头获取的组织明显更多（中位数重量差异为1.5毫克，95%置信区间为0.8 - 2.3毫克）。使用较大的20毫升注射器也能获取更多组织，不过注射器尺寸的影响小于针头尺寸（中位数差异为0.8毫克，95%置信区间为0 - 1.6）。
较大尺寸的注射器和针头能从足月产后胎盘中获取更多数量的绒毛膜绒毛。
