Percherancier Yann, Goudeau Bertrand, Charlet de Sauvage Renaud, de Gannes Florence Poulletier, Haro Emmanuelle, Hurtier Annabelle, Sojic Neso, Lagroye Isabelle, Arbault Stéphane, Veyret Bernard
IMS Laboratory, University of Bordeaux, CNRS, Talence, France.
Bioelectromagnetics. 2015 Apr;36(4):287-93. doi: 10.1002/bem.21908. Epub 2015 Apr 3.
The present study focused on gap junctional intercellular communication (GJIC) as a target for biological effects of extremely low-frequency (ELF) magnetic field (MF) exposure. Fluorescence recovery after photobleaching microscopy (FRAP) was used to visualize diffusion of a fluorescent dye between NIH3T3 fibroblasts through gap junctions. The direct effect of 24 h exposure to 50 Hz MF at 0.4 or 1 mT on GJIC function was assessed in one series of experiments. The potential synergism of MF with an inhibitor of GJIC, phorbol ester (TPA), was studied in another series by observing FRAP when NIH3T3 cells were incubated with TPA for 1 h following 24 h exposure to MF. In contrast to other reports of ELF-MF effects on GJIC, under our experimental conditions we observed neither direct inhibition of GJIC nor synergism with TPA-induced inhibition from 50 Hz MF exposures.
本研究聚焦于缝隙连接细胞间通讯（GJIC），将其作为极低频（ELF）磁场（MF）暴露生物效应的一个靶点。光漂白后荧光恢复显微镜技术（FRAP）用于可视化荧光染料在NIH3T3成纤维细胞间通过缝隙连接的扩散。在一系列实验中评估了0.4或1 mT的50 Hz MF暴露24小时对GJIC功能的直接影响。在另一系列实验中，通过观察NIH3T3细胞在暴露于MF 24小时后与佛波酯（TPA）孵育1小时时的FRAP，研究了MF与GJIC抑制剂佛波酯（TPA）的潜在协同作用。与其他关于ELF-MF对GJIC影响的报道相反，在我们的实验条件下，我们既未观察到对GJIC的直接抑制，也未观察到50 Hz MF暴露与TPA诱导的抑制之间的协同作用。
