Waksmundzka-Hajnos Monika, Petruczynik Anna, Dragan Anna, Wianowska Dorota, Dawidowicz Andrzej L, Sowa Ireneusz
Department of Inorganic and Analytical Chemistry, Medical University, Staszica 6, 20-081 Lublin, Poland.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Feb 5;800(1-2):181-7. doi: 10.1016/j.jchromb.2003.07.006.
Analysis of plant material is an important task in chemotaxonomical investigations, in search of plants with pharmacological activity or in standardisation of plant drugs. The choice of optimal conditions for the analysis of plant material and effect of extraction method on the yield of furanocoumarins from Pastinaca sativa fruits were examined. The following extraction methods were used in experiments: exhaustive extraction in Soxhlet apparatus, ultrasonification (USAE) at 25 and 60 degrees C, microwave-assisted solvent extraction in open and closed system (MASE) and accelerated solvent extraction (ASE). In most cases, the yield of furanocoumarins was highest by use of ASE method as well as by ultrasonification at 60 degrees C.
植物材料分析是化学分类学研究、寻找具有药理活性的植物或植物药标准化中的一项重要任务。研究了分析植物材料的最佳条件选择以及提取方法对欧防风果实中呋喃香豆素产量的影响。实验中采用了以下提取方法：索氏提取器中的 exhaustive 提取、25℃和60℃下的超声提取（USAE）、开放和封闭系统中的微波辅助溶剂提取（MASE）以及加速溶剂提取（ASE）。在大多数情况下，使用ASE方法以及在60℃下超声提取时，呋喃香豆素的产量最高。
