Department of Pharmacognosy with Medicinal Plant Unit, Medical University in Lublin, 1 Chodzki, Lublin 20-093, Poland.
Molecules. 2012 Apr 5;17(4):4133-41. doi: 10.3390/molecules17044133.
Coumarins are nowadays an important group of organic compounds from natural sources that are useful in a number of fields. Because they possess different pharmacological properties, finding the proper extraction conditions for their separation from plant matrices is a very important step. In this report Pressurized Liquid Extraction (PLE) under different temperature conditions and with different types of extraction solvents were tested. As a matrix, fruits of Heracleum leskowii have been used. A simple reverse phase high-performance liquid chromatographic method (RP-HPLC) coupled with a photodiode array detector (DAD) has been developed for separation and quantitative analysis of the main coumarins. Umbelliferone, xanthotoxin, angelicin, isopimpinellin, bergapten, imperatorin and isoimperatorin were investigated. Bergapten and imperatorin were dominant in almost all extracts in the range of 9.92 ± 0.02-20.93 ± 0.06 and 12.19 ± 0.98-19.07 ± 0.03 mg/100 g, respectively. Dichloromethane and methanol were chosen as the most proper suitable solvents for extraction of coumarins. By increasing the temperature the amount of extracted coumarins increases in petroleum ether and dichloromethane extracts.
香豆素是现今一类重要的天然来源有机化合物，在多个领域都有应用。由于它们具有不同的药理特性，因此找到从植物基质中分离它们的合适提取条件是非常重要的一步。在本报告中，测试了不同温度条件下使用不同类型提取溶剂的加压液体萃取（PLE）。作为基质，使用了独活属植物果实。开发了一种简单的反相高效液相色谱法（RP-HPLC）与光电二极管阵列检测器（DAD）相结合的方法，用于主要香豆素的分离和定量分析。对 Umbelliferone、xanthotoxin、angelicin、isopimpinellin、bergapten、imperatorin 和 isoimperatorin 进行了研究。bergapten 和 imperatorin 在几乎所有提取物中均占主导地位，范围在 9.92 ± 0.02-20.93 ± 0.06 和 12.19 ± 0.98-19.07 ± 0.03 mg/100 g 之间。二氯甲烷和甲醇被选为提取香豆素的最适宜溶剂。通过提高温度，石油醚和二氯甲烷提取物中提取的香豆素的量增加。
