Kalaivanisailaja Jawaharbabu, Manju Vaiyapuri, Nalini Namasivayam
Department of Biochemistry, Faculty of Science, Annamalai University, Annamalainagar - 608 002, Tamil Nadu, India.
Pol J Pharmacol. 2003 Sep-Oct;55(5):763-9.
Previous studies suggest a possible link between leptin and decreased lipid levels, however, the role of leptin in high-fat diet-induced hyperlipidemia remains unclear. The aim of our study was to evaluate the effect of administering leptin on plasma and tissue lipids in mice fed a high-fat diet. Feeding a high-fat diet (2% cholesterol, 0.125% bile salts, 5% peanut oil) to four-week-old healthy mice for a period of 45 days, resulted in significantly elevated levels of plasma and tissue total cholesterol, phospholipids, free fatty acids and triglycerides as compared with those of the control mice. Subsequently after thirty days, exogenous leptin (230 microg/kg i.p.) was administered simultaneously with the daily dose of high-fat diet every alternate day for fifteen days. Leptin administration significantly reduced the levels of total cholesterol, phospholipids, free fatty acids and triglycerides in the plasma, liver, heart and kidney of both the control and high-fat diet fed mice. Moreover, leptin administration markedly reduced the levels of plasma LDL, VLDL and elevated plasma HDL and the activity of lipoprotein lipase as compared with the untreated control and high-fat diet fed mice. Thus, leptin administration was found to have a marked protective effect against hyperlipidemia and thus obesity, by virtue of its lipid lowering effects.
先前的研究表明瘦素与血脂水平降低之间可能存在联系，然而，瘦素在高脂饮食诱导的高脂血症中的作用仍不清楚。我们研究的目的是评估给予瘦素对高脂饮食喂养小鼠的血浆和组织脂质的影响。给四周龄健康小鼠喂食高脂饮食（2%胆固醇、0.125%胆盐、5%花生油）45天，与对照小鼠相比，血浆和组织中的总胆固醇、磷脂、游离脂肪酸和甘油三酯水平显著升高。随后在30天后，每隔一天在给予每日高脂饮食剂量的同时给予外源性瘦素（230微克/千克腹腔注射），持续15天。给予瘦素显著降低了对照小鼠和高脂饮食喂养小鼠的血浆、肝脏、心脏和肾脏中的总胆固醇、磷脂、游离脂肪酸和甘油三酯水平。此外，与未治疗的对照小鼠和高脂饮食喂养小鼠相比，给予瘦素显著降低了血浆低密度脂蛋白、极低密度脂蛋白水平，提高了血浆高密度脂蛋白水平和脂蛋白脂肪酶的活性。因此，由于其降血脂作用，发现给予瘦素对高脂血症进而对肥胖具有显著的保护作用。
