Park Dae-Won, Haam Seungjoo, Ahn Ik-Sung, Lee Tai Gyu, Kim Hae-Sung, Kim Woo-Sik
Department of Chemical Engineering, College of Engineering, Yonsei University, Seoul, South Korea.
J Biotechnol. 2004 Jan 22;107(2):151-60. doi: 10.1016/j.jbiotec.2003.09.004.
The enzymatic esterifications of beta-methylglucoside with acrylic acid/methacrylic acid were carried out using Novozym 435. t-Butanol indicating the highest conversion value was determined as an optimal solvent. The molar ratio (beta-methylglucoside:acids) of 1:15 was most favorable to the esterification. The enzyme concentration of 5% (w/v), and the temperature (50 degrees C for beta-methylglucoside:acrylic acid, 45 degrees C for beta-methylglucoside:methacrylic acid) resulted in the highest final conversion. Beta-methylglucoside of 60gl(-1) was found to be most effective in terms of short reaction time as well as product concentrations. Under these conditions, the maximum conversions for the esterification of beta-methylglucoside with acrylic acid and beta-methylglucoside with methacrylic acid were 59.3% after 12h and 71.3% after 72h, respectively. The structural analysis of the products was performed by FT-IR spectroscopy and (1)H NMR.
