微波辅助固定化脂肪酶 Novozym 435 合成 5'-甲丙烯酰胞苷
Microwave-Assisted Synthesis of 5'--methacryloylcytidine Using the Immobilized Lipase Novozym 435.
机构信息
Fraunhofer Institute of Applied Polymer Research, Biofunctionalized Materials and (Glyco) Biotechnology, Geiselbergstr. 69, 14476 Potsdam, Germany.
Chair of Polymer Materials and Polymer Technologies, Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam, Germany.
出版信息
Molecules. 2022 Jun 26;27(13):4112. doi: 10.3390/molecules27134112.
Nucleobase building blocks have been demonstrated to be strong candidates when it comes to DNA/RNA-like materials by benefiting from hydrogen bond interactions as physical properties. Modifying at the 5' position is the simplest way to develop nucleobase-based structures by transesterification using the lipase Novozym 435. Herein, we describe the optimization of the lipase-catalyzed synthesis of the monomer 5'--methacryloylcytidine with the assistance of microwave irradiation. Variable reaction parameters, such as enzyme concentration, molar ratio of the substrate, reaction temperature and reaction time, were investigated to find the optimum reaction condition in terms of obtaining the highest yield.
核苷碱基砌块在受益于氢键相互作用等物理性质时，已被证明是 DNA/RNA 样材料的有力候选物。通过使用脂肪酶 Novozym 435 进行酯交换反应，在 5'位置进行修饰是开发基于核苷碱基的结构的最简单方法。在此，我们描述了在微波辐射的辅助下，通过脂肪酶催化合成单体 5'-甲丙烯酰胞苷的优化。考察了可变反应参数，如酶浓度、底物的摩尔比、反应温度和反应时间，以找到获得最高产率的最佳反应条件。
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