Bozdogan Bülent, Galopin Sébastien, Leclercq Roland
Service de Microbiologie, CHU Cote de Nacre, Caen, France.
Appl Environ Microbiol. 2004 Jan;70(1):280-4. doi: 10.1128/AEM.70.1.280-284.2004.
The mechanism of resistance to macrolides, lincosamides, and streptogramins B was studied in four Bacillus clausii strains that are mixed in a probiotic administered to humans for prevention of gastrointestinal side effects due to oral antibiotic chemotherapy and in three reference strains of B. clausii, DSM8716, ATCC 21536, and ATCC 21537. An 846-bp gene called erm(34), which is related to the erm genes conferring resistance to these antibiotics by ribosomal methylation, was cloned from total DNA of B. clausii DSM8716 into Escherichia coli. The deduced amino acid sequence presented 61% identity with that of Erm(D) from B. licheniformis, B. halodurans, and B. anthracis. Pulsed-field gel electrophoresis of total DNA digested by I-CeuI, followed by hybridization with an erm(34)-specific probe, indicated a chromosomal location of the gene in all B. clausii strains. Repeated attempts to transfer resistance to macrolides by conjugation from B. clausii strains to Enterococcus faecalis JH2-2, E. faecium HM1070, and B. subtilis UCN19 were unsuccessful.
对四种克劳氏芽孢杆菌菌株进行了研究，这四种菌株混合在一种用于预防口服抗生素化疗引起的胃肠道副作用的益生菌中，同时还研究了三种克劳氏芽孢杆菌参考菌株DSM8716、ATCC 21536和ATCC 21537对大环内酯类、林可酰胺类和链阳菌素B的耐药机制。从克劳氏芽孢杆菌DSM8716的总DNA中克隆出一个846 bp的基因erm(34)，该基因与通过核糖体甲基化赋予对这些抗生素耐药性的erm基因相关，并将其导入大肠杆菌。推导的氨基酸序列与来自地衣芽孢杆菌、嗜碱芽孢杆菌和炭疽芽孢杆菌的Erm(D)的氨基酸序列具有61%的同一性。用I-CeuI消化总DNA，然后用erm(34)特异性探针进行杂交的脉冲场凝胶电泳表明，该基因在所有克劳氏芽孢杆菌菌株中均位于染色体上。多次尝试通过接合作用将克劳氏芽孢杆菌菌株对大环内酯类的耐药性转移至粪肠球菌JH2-2、屎肠球菌HM1070和枯草芽孢杆菌UCN19均未成功。
