Wong Fai-Chu, Yong Ann-Li, Ting Evon Peir-Shan, Khoo Sim-Chyi, Ong Hean-Chooi, Chai Tsun-Thai
Centre for Biodiversity Research, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia. ; Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia.
Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia.
Iran J Pharm Res. 2014 Fall;13(4):1409-15.
The purpose of this investigation was to determine the antioxidant potentials and anti-glucosidase activities of six tropical medicinal plants. The levels of phenolic constituents in these medicinal plants were also quantified and compared. Antioxidation potentials were determined colorimetrically for scavenging activities against DPPH and NO radicals. Metal chelating assay was based on the measurement of iron-ferrozine absorbance at 562 nm. Anti-diabetic potentials were measured by using α-glucosidase as target enzyme. Medicinal plants' total phenolic, total flavonoid and hydroxycinnamic acid contents were determined using spectrophotometric methods, by comparison to standard plots prepared using gallic acid, quercetin and caffeic acid standards, respectively. Radical scavenging and metal chelating activities were detected in all medicinal plants, in concentration-dependent manners. Among the six plants tested, C. nutans, C. formosana and H. diffusa were found to possess α-glucosidase inhibitory activities. Spectrophotometric analysis indicated that the total phenolic, total flavonoid and hydroxycinnamic acid contents ranged from 12.13-21.39 mg GAE per g of dry sample, 1.83-9.86 mg QE per g of dry sample, and 0.91-2.74 mg CAE per g of dry sample, respectively. Our results suggested that C. nutans and C. formosana could potentially be used for the isolation of potent antioxidants and anti-diabetic compounds. To the best of our knowledge, this study represents the first time that C. nutans (Acanthaceae family) was reported in literature with glucosidase inhibition activity.
本研究的目的是测定六种热带药用植物的抗氧化潜力和抗葡萄糖苷酶活性。还对这些药用植物中酚类成分的含量进行了定量和比较。采用比色法测定抗氧化潜力，以评估对DPPH和NO自由基的清除活性。金属螯合试验基于测定562nm处铁-菲咯嗪的吸光度。以α-葡萄糖苷酶为靶酶测定抗糖尿病潜力。通过分光光度法分别与使用没食子酸、槲皮素和咖啡酸标准品制备的标准曲线比较，测定药用植物的总酚、总黄酮和羟基肉桂酸含量。所有药用植物均检测到自由基清除和金属螯合活性，且呈浓度依赖性。在所测试的六种植物中，发现垂花悬铃花、台湾悬铃花和白花蛇舌草具有α-葡萄糖苷酶抑制活性。分光光度分析表明，总酚、总黄酮和羟基肉桂酸含量分别为每克干样品12.13-21.39mg没食子酸当量、每克干样品1.83-9.86mg槲皮素当量和每克干样品0.91-2.74mg咖啡酸当量。我们的结果表明，垂花悬铃花和台湾悬铃花可能可用于分离强效抗氧化剂和抗糖尿病化合物。据我们所知，本研究首次在文献中报道了垂花悬铃花（爵床科）具有葡萄糖苷酶抑制活性。
