Aspenberg P, Wang E, Thorngren K G
Department of Orthopedics, Lund University Hospital, Sweden.
Acta Orthop Scand. 1992 Dec;63(6):619-22.
Demineralized bone matrix (DBM) reproducibly induces extraskeletal bone formation in rodents, but its effects in dogs and primates are negative or uncertain. In previous studies on the squirrel monkey, DBM did not induce bone, although the same implants were effective in nude rats. In the present study, the DBM was augmented with recombinant human bone morphogenetic protein-2 (BMP-2). Bone was formed in 10 of 12 monkeys, as verified by histology and calcium content. However, in 4 monkeys, the induced bone mass appeared smaller than the original implant. DBM controls induced microscopic amounts of bone in 2 out of 10 monkeys. In the nude rats, all DBM controls and augmented implants induced bone. The difficulties in achieving bone induction in higher animals may be overcome, at least partially, by using a higher concentration of the inductive protein than is present in DBM.
脱矿骨基质（DBM）可在啮齿动物中反复诱导骨骼外骨形成，但其在犬类和灵长类动物中的作用为阴性或不确定。在先前对松鼠猴的研究中，DBM未诱导出骨形成，尽管相同的植入物在裸鼠中有效。在本研究中，DBM用重组人骨形态发生蛋白-2（BMP-2）进行了增强。经组织学和钙含量验证，12只猴子中有10只形成了骨。然而，在4只猴子中，诱导的骨量似乎比原始植入物小。DBM对照组在10只猴子中有2只诱导出微量的骨。在裸鼠中，所有DBM对照组和增强植入物均诱导出骨。通过使用比DBM中存在的诱导蛋白更高的浓度，至少可以部分克服在高等动物中实现骨诱导的困难。
