Diniz Yeda S, Cicogna Antonio C, Padovani Carlos R, Silva Maeli D P, Faine Luciane A, Galhardi Cristiano M, Rodrigues Hosana G, Novelli Ethel L B
Faculty of Medicine, UNESP, Botucatu, São Paulo, Brazil.
Can J Physiol Pharmacol. 2003 Nov;81(11):1042-8. doi: 10.1139/y03-097.
Dietary modification ought to be the first line of strategy in prevention of the development of cardiac disease. The purpose of this study was to investigate whether dietary restriction, dietary-fibre-enriched diet, and their interactions might affect antioxidant capacity and oxidative stress in cardiac tissue. Male Wistar rats (180-200 g; n=10) were divided into four groups: control ad libitum diet (C), 50% restricted diet (DR), fed with fibre-enriched diet (F), and 50% restricted fibre-enriched diet (DR-F). After 35 days of the treatments, F, DR, and DR-F rats showed low cholesterol, LDL-cholesterol, and triacylglycerol, and high HDL-cholesterol in serum. The DR, DR-F, and F groups had decreased myocardial lipoperoxide and lipid hydroperoxide. The DR-F and F treatments increased superoxide dismutase and glutatione peroxidase (GSH-Px). The DR treatment increased GSH-Px and catalase activities. Dietary fibre beneficial effects were related to metabolic alterations. The F and DR-F groups showed high cardiac glycogen and low lactate dehydrogenase/citrate synthase ratios, indicating diminished anaerobic and elevated aerobic myocardial metabolism in these animals. There was no synergistic effect between dietary restriction and dietary fibre addition, since no differences were observed in markers of oxidative stress in the F and DR-F groups. Dietary fibre supplementation, rather than energy intake and dietary restriction, appears to be the main process retarding oxidative stress in cardiac tissue.
饮食调整应成为预防心脏病发生的首要策略。本研究的目的是调查饮食限制、富含膳食纤维的饮食及其相互作用是否会影响心脏组织中的抗氧化能力和氧化应激。将雄性Wistar大鼠（180 - 200克；n = 10）分为四组：自由摄食对照组（C）、50%限制饮食组（DR）、喂食富含纤维饮食组（F）和50%限制富含纤维饮食组（DR - F）。治疗35天后，F组、DR组和DR - F组大鼠血清中的胆固醇、低密度脂蛋白胆固醇和三酰甘油水平较低，而高密度脂蛋白胆固醇水平较高。DR组、DR - F组和F组的心肌脂过氧化物和脂质氢过氧化物减少。DR - F组和F组的治疗增加了超氧化物歧化酶和谷胱甘肽过氧化物酶（GSH - Px）。DR组的治疗增加了GSH - Px和过氧化氢酶的活性。膳食纤维的有益作用与代谢改变有关。F组和DR - F组显示心脏糖原含量高，乳酸脱氢酶/柠檬酸合酶比值低，表明这些动物的无氧心肌代谢减少，有氧心肌代谢增强。饮食限制和添加膳食纤维之间没有协同作用，因为在F组和DR - F组的氧化应激标志物中未观察到差异。补充膳食纤维，而非能量摄入和饮食限制，似乎是减缓心脏组织氧化应激的主要过程。
