Fenech Marianne, Jaffrin Michel Y
Department of Biological Engineering, Technological University of Compiegne, 60205 Compiègne Cedex, France.
IEEE Trans Biomed Eng. 2004 Jan;51(1):166-75. doi: 10.1109/TBME.2003.820338.
Extracellular (ECW) and intracellular (ICW) volumes were measured using both segmental and wrist-ankle (W-A) bioimpedance spectroscopy (5-1000 kHz) in 15 healthy subjects (7 men, 8 women). In the 1st protocol, the subject, after sitting for 30 min, laid supine for at least 30 min. In the second protocol, the subject, who had been supine for 1 hr, sat up in bed for 10 min and returned to supine position for another hour. Segmental ECW and ICW resistances of legs, arms and trunk were measured by placing four voltage electrodes on wrist, shoulder, top of thigh and ankle and using Hanai's conductivity theory. W-A resistances were found to be very close to the sum of segmental resistances. When switching from sitting to supine (protocol 1), the mean ECW leg resistance increased by 18.2%, that of arm and W-A by 12.4%. Trunk resistance also increased but not significantly by 4.8%. Corresponding increases in ICW resistance were smaller for legs (3.7%) and arm (-0.7%) but larger for the trunk (21.4%). Total body ECW volumes from segmental measurements were in good agreement with W-A and Watson anthropomorphic correlation. The decrease in total ECW volume (when supine) calculated from segmental resistances was at 0.79 l less than the W-A one (1.12 l). Total ICW volume reductions were 3.4% (segmental) and 3.8% (W-A). Tests of protocol 2 confirmed that resistance and fluid volume values were not affected by a temporary position change.
在15名健康受试者（7名男性，8名女性）中，使用分段和腕踝（W-A）生物电阻抗光谱法（5-1000kHz）测量细胞外液（ECW）和细胞内液（ICW）体积。在第一个方案中，受试者静坐30分钟后，仰卧至少30分钟。在第二个方案中，已经仰卧1小时的受试者在床上坐起10分钟，然后再仰卧1小时。通过在手腕、肩部、大腿顶部和脚踝处放置四个电压电极，并使用花井的电导率理论，测量腿部、手臂和躯干的分段ECW和ICW电阻。发现W-A电阻非常接近分段电阻之和。从坐姿转换为仰卧姿（方案1）时，腿部的平均ECW电阻增加了18.2%，手臂和W-A的平均ECW电阻增加了12.4%。躯干电阻也增加了，但不显著，为4.8%。腿部（3.7%）和手臂（-0.7%）的ICW电阻相应增加较小，但躯干的ICW电阻增加较大（21.4%）。分段测量得到的全身ECW体积与W-A和沃森人体测量学相关性良好。根据分段电阻计算的仰卧时总ECW体积减少量比W-A测量值少0.79升（W-A测量值为1.12升）。总ICW体积减少量分别为3.4%（分段测量）和3.8%（W-A测量）。方案2的测试证实，电阻和液体体积值不受临时体位变化的影响。
