Tonouchi Naoto, Sugiyama Masakazu, Yokozeki Kenzo
AminoScience Laboratories, Ajinomoto Co., Inc., Kawasaki-shi, Japan.
Biosci Biotechnol Biochem. 2003 Dec;67(12):2648-51. doi: 10.1271/bbb.67.2648.
The coenzyme specificity of enzymes in the oxidative pentose phosphate pathway of Gluconobacter oxydans was investigated. By investigation of the activities of glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) in the soluble fraction of G. oxydans, and cloning and expression of genes in Escherichia coli, it was found that both G6PDH and 6PGDH have NAD/NADP dual coenzyme specificities. It was suggested that the pentose phosphate pathway is responsible for NADH regeneration in G. oxydans.
研究了氧化葡萄糖杆菌磷酸戊糖途径中酶的辅酶特异性。通过对氧化葡萄糖杆菌可溶性部分中葡萄糖-6-磷酸脱氢酶（G6PDH）和6-磷酸葡萄糖酸脱氢酶（6PGDH）活性的研究，以及在大肠杆菌中的基因克隆和表达，发现G6PDH和6PGDH都具有NAD/NADP双辅酶特异性。有人提出磷酸戊糖途径负责氧化葡萄糖杆菌中NADH的再生。
