Metges Cornelia C, Lehmann Lutz, Boeuf Stephane, Petzke Klaus J, Müller André, Rickert Rainer, Franke Wittko, Steinhart Hans, Nürnberg Gerd, Klaus Susanne
German Institute of Human Nutrition, 14558 Bergholz-Rehbrücke, Germany.
Lipids. 2003 Nov;38(11):1133-42. doi: 10.1007/s11745-003-1171-0.
We explored whether CLA isomers and other C18 FA affect (i) lipid content and FA concentrations in total adipocyte lipids, (ii) FA synthesis from glucose in TAG and phospholipids of primary brown (BAT) and white adipocytes (WAT), and (iii) mRNA expression of uncoupling protein 1 (UCP1) in primary brown adipocytes of Djungarian hamsters (Phodopus sungorus). c9,t11-CLA, oleic, linoleic, and alpha-linolenic acid increased whereas t10,c12-CLA decreased lipid accumulation in both adipocyte types. t10,c12-CLA treatment affected FA composition mainly in BAT cells. CLA incorporation into lipids, in particular c9,t11-CLA, was higher in BAT. In both cell types, t10,c12-CLA treatment reduced the incorporation of glucose 13C carbon into FA of TAG and phospholipids, whereas c9,t11-CLA, linoleic, and alpha-linolenic acid either did not influence or dose-dependently increased glucose carbon incorporation into FA. UCP1 mRNA expression was inhibited by t10,c12-CLA but increased by c9,t11-CLA, linoleic, and alpha-linolenic acid. It is concluded that c9,t11-CLA and t10,c12-CLA have distinctly different effects on lipid metabolism in primary adipocytes. The effects of c9,t11-CLA are similar to those of other unsaturated C18 FA. The opposite effects of c9,t11-CLA and t10,c12-CLA are evident in both WAT and BAT cultures; however, brown adipocytes seem to be more susceptible to CLA treatment.
我们探究了共轭亚油酸（CLA）异构体和其他C18脂肪酸是否会影响：（i）总脂肪细胞脂质中的脂质含量和脂肪酸浓度；（ii）原代棕色脂肪细胞（BAT）和白色脂肪细胞（WAT）中三酰甘油（TAG）和磷脂中由葡萄糖合成脂肪酸的过程；以及（iii）黑线毛足鼠（Phodopus sungorus）原代棕色脂肪细胞中解偶联蛋白1（UCP1）的mRNA表达。顺-9，反-11-CLA、油酸、亚油酸和α-亚麻酸增加了两种脂肪细胞类型中的脂质积累，而反-10，顺-12-CLA则减少了脂质积累。反-10，顺-12-CLA处理主要影响BAT细胞中的脂肪酸组成。CLA掺入脂质中，尤其是顺-9，反-11-CLA，在BAT中更高。在两种细胞类型中反-10，顺-12-CLA处理均降低了葡萄糖13C碳掺入TAG和磷脂脂肪酸中的比例，而顺-9，反-11-CLA、亚油酸和α-亚麻酸要么没有影响，要么剂量依赖性地增加了葡萄糖碳掺入脂肪酸中的比例。反-10，顺-12-CLA抑制UCP1 mRNA表达，而顺-9，反-11-CLA、亚油酸和α-亚麻酸则增加UCP1 mRNA表达。得出的结论是，顺-9，反-11-CLA和反-10，顺-12-CLA对原代脂肪细胞脂质代谢具有明显不同的影响。顺-9，反-11-CLA的作用与其他不饱和C18脂肪酸相似。顺-9，反-11-CLA和反-10，顺-12-CLA的相反作用在WAT和BAT培养物中均很明显；然而，棕色脂肪细胞似乎对CLA处理更敏感。
