Alquini Giovana, Carbonero Elaine R, Rosado Fábio R, Cosentino Cesare, Iacomini Marcello
Departamento de Bioquímica, Universidade Federal do Paraná, Curitiba-PR, Brazil.
FEMS Microbiol Lett. 2004 Jan 15;230(1):47-52. doi: 10.1016/S0378-1097(03)00853-X.
The two main polysaccharides from the basidiomycetous fungus Laetiporus sulphureus were isolated, purified and characterized. The structural assignments were carried out using (13)C, (1)H, and (1)H,(13) HSQC nuclear magnetic resonance spectroscopy, methylation analysis, and Smith degradation. One was a linear beta-glucan having a (1-->3)-linked main chain, namely laminaran. The other was a fucomannogalactan, which consisted of a main chain of (1-->6)-linked alpha-D-galactopyranosyl residues, a part of them being substituted at O-2 by 3-O-D-mannopyranosyl-L-fucopyranosyl, alpha-D-mannopyranosyl and in a minor proportion, alpha-L-fucopyranosyl groups. This heteropolysaccharide is related to those of other Basidiomycetes heterogalactans, although it differs distinctly in its side-chain structures. Whereas part of the single-unit L-fucopyranosyl and/or 3-O-alpha-mannopyranosyl-L-fucopyranosyl residues are present as side chains of the other heterogalactans, additional alpha-D-mannopyranosyl units are present in our fucomannogalactan of L. sulphureus.
从担子菌硫磺多孔菌中分离、纯化并鉴定了两种主要的多糖。使用碳-13、氢-1和氢-1,碳-13异核单量子相干核磁共振光谱、甲基化分析和史密斯降解法进行结构鉴定。一种是具有(1→3)连接主链的线性β-葡聚糖，即海带多糖。另一种是岩藻甘露半乳聚糖，它由(1→6)连接的α-D-吡喃半乳糖基残基的主链组成，其中一部分在O-2位被3-O-D-吡喃甘露糖基-L-岩藻糖基、α-D-吡喃甘露糖基取代，还有一小部分被α-L-岩藻糖基取代。这种杂多糖与其他担子菌杂半乳聚糖有关，尽管其侧链结构明显不同。其他杂半乳聚糖的侧链中存在部分单单元L-岩藻糖基和/或3-O-α-吡喃甘露糖基-L-岩藻糖基残基，而在我们的硫磺多孔菌岩藻甘露半乳聚糖中还存在额外的α-D-吡喃甘露糖基单元。
