Williams Philip G, Yoshida Wesley Y, Moore Richard E, Paul Valerie J
Department of Chemistry, University of Hawaii at Manoa, Honolulu, HI 96822, USA.
J Nat Prod. 2004 Jan;67(1):49-53. doi: 10.1021/np030215x.
Two new cytotoxins have been isolated from a species of marine cyanobacterium belonging to the genus Symploca that was collected in Guam. These new compounds, micromide (1) and guamamide (2), were accompanied by the known lipopeptides apramides A (3), B (4), and G (5). The planar structures of both alkaloids were elucidated by standard 2D NMR techniques, and the configurations of the amino acid-derived units in 1 were determined by chiral HPLC. The stereochemistry of the beta-methoxyhexanoic acid in 1 was determined by derivatization with methyl d-mandelate, after acid hydrolysis, and comparison with synthetic standards.
从关岛采集的一种席藻属海洋蓝藻中分离出了两种新的细胞毒素。这些新化合物，即微酰胺（1）和关岛酰胺（2），与已知的脂肽阿普拉酰胺A（3）、B（4）和G（5）一同被发现。两种生物碱的平面结构通过标准二维核磁共振技术得以阐明，化合物1中氨基酸衍生单元的构型通过手性高效液相色谱法确定。化合物1中β-甲氧基己酸的立体化学结构是在酸水解后用d-扁桃酸甲酯进行衍生化，并与合成标准品进行比较后确定的。
