Rosa-Freitas M G, Broomfield G, Priestman A, Milligan P J, Momen H, Molyneux D H
Departamento de Bioquimica e Biologia Molecular, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.
J Am Mosq Control Assoc. 1992 Dec;8(4):357-66.
Three populations of Anopheles darlingi were studied for cuticular hydrocarbons, isoenzymes and patterns of peak biting activity. Differences were found in specimens from Costa Marques, a malaria endemic area; Dourado, a site with a very exophilic population and Juturnaíba, located near the type locality. Twelve hour collections from sunset to sunrise showed that An. darlingi from Costa Marques had a bimodal biting activity profile with a major peak at sunset and a minor peak at sunrise. At Dourado, the pattern was trimodal, with peaks at both morning and evening periods of twilight and near midnight. The Juturnaíba population showed a slight increase in activity near 2000 and 0100 h. Nei's genetic distances, determined by isoenzyme electrophoresis between pairs of populations, were low (D < or = 0.049). Using discriminant analysis for the cuticular hydrocarbons, 92.4% of the specimens from Costa Marques, 91.2% of the specimens from Dourado and 61.3% from Juturnaíba were correctly identified. Cuticular hydrocarbon and isoenzyme results matched very well: the smaller the Nei's distance, the more misidentifications occurred in the jackknife estimator used in the cuticular hydrocarbon analysis. This is the first report of cuticular hydrocarbon analysis in combination with isoenzymes to investigate neotropical anopheline species.
对三个达林按蚊种群的表皮碳氢化合物、同工酶及高峰叮咬活动模式进行了研究。在来自疟疾流行区科斯塔马克斯、具有非常嗜外习性种群的多拉多以及位于模式产地附近的茹图尔奈巴的标本中发现了差异。从日落到日出进行的12小时采集显示，来自科斯塔马克斯的达林按蚊具有双峰叮咬活动模式，在日落时出现一个主要高峰，在日出时出现一个次要高峰。在多拉多，模式为三峰型，在黎明的早晨和傍晚时段以及接近午夜时出现高峰。茹图尔奈巴种群在2000时和0100时附近活动略有增加。通过对种群对之间进行同工酶电泳测定的内氏遗传距离较低（D≤0.049）。利用表皮碳氢化合物的判别分析，来自科斯塔马克斯的92.4%的标本、来自多拉多的91.2%的标本以及来自茹图尔奈巴的61.3%的标本被正确识别。表皮碳氢化合物和同工酶结果非常吻合：内氏距离越小，在表皮碳氢化合物分析中使用的刀切估计器中出现的错误识别就越多。这是将表皮碳氢化合物分析与同工酶相结合以研究新热带区按蚊种类的首次报告。
