膳食抗癌异硫氰酸盐在细胞内积累后，会通过转运蛋白介导以二硫代氨基甲酸盐的形式输出。

Cellular accumulation of dietary anticarcinogenic isothiocyanates is followed by transporter-mediated export as dithiocarbamates.

作者信息

Callaway Eileen C, Zhang Yuesheng, Chew Wade, Chow H-H Sherry

机构信息

Arizona Cancer Center, College of Medicine, University of Arizona, Tucson, AZ 85724, USA.

出版信息

Cancer Lett. 2004 Feb 10;204(1):23-31. doi: 10.1016/j.canlet.2003.09.021.

DOI:10.1016/j.canlet.2003.09.021

PMID:14744531

Abstract

Many dietary isothiocyanates (ITCs) are potent anticarcinogenic agents. ITCs rapidly accumulate to high concentrations in cells as a result of conjugation with intracellular thiols, especially glutathione (GSH). The anticarcinogenic activity of ITCs depends on, at least partly, their accumulation in cells. We report that three major anticarcinogenic ITCs, including allyl-ITC, benzyl-ITC, and phenethyl-ITC, were rapidly exported, upon accumulation in cells, mainly in the forms of GSH- and cysteinylglycine-conjugates, apparently involving MRP-1 and Pgp-1. These findings are consistent with our previous results regarding cellular export of another anticarcinogenic ITC, sulforaphane, and suggest a common cellular response to ITCs.

摘要

许多膳食异硫氰酸盐（ITC）是强效抗癌剂。由于与细胞内硫醇，尤其是谷胱甘肽（GSH）结合，ITC在细胞中迅速积累至高浓度。ITC的抗癌活性至少部分取决于它们在细胞中的积累。我们报告，三种主要的抗癌ITC，包括烯丙基-ITC、苄基-ITC和苯乙基-ITC，在细胞中积累后迅速被排出，主要以GSH和半胱氨酰甘氨酸缀合物的形式，显然涉及多药耐药相关蛋白1（MRP-1）和P-糖蛋白1（Pgp-1）。这些发现与我们之前关于另一种抗癌ITC萝卜硫素细胞排出的结果一致，并提示细胞对ITC有共同反应。