Gaede Holly C, Gawrisch Klaus
Laboratory of Membrane Biochemistry and Biophysics, NIAAA, NIH, 12420 Parklawn Drive, Rockville, Maryland 20852, USA.
Magn Reson Chem. 2004 Feb;42(2):115-22. doi: 10.1002/mrc.1329.
The benefits of gradient techniques in the study of lipid membranes are demonstrated on a sample of 1-palmitoyl-2-oleoyl-sn-glycero-3 phosphocholine (POPC) liposomes embedded with ibuprofen. Most techniques from gradient NMR spectroscopy on solution samples are directly applicable to membrane samples subjected to magic angle spinning (MAS). Gradient-enhanced homo- and heteronuclear chemical shift correlation techniques were used to make resonance assignments. Gradient NOESY experiments provide insight into the location and dynamics of lipids, ibuprofen and water. Application of gradients not only reduces experiment time but also the t(1) noise in the multi-dimensional spectra. Diffusion measurements with pulsed field gradients characterize lateral movements of lipid and drug molecules in membranes. The theoretical framework for data analysis of MAS diffusion experiments on randomly oriented multilamellar liposomes is presented.
在嵌入布洛芬的1-棕榈酰-2-油酰基-sn-甘油-3-磷酸胆碱（POPC）脂质体样品上，展示了梯度技术在脂质膜研究中的优势。溶液样品的梯度核磁共振光谱法中的大多数技术都可直接应用于经受魔角旋转（MAS）的膜样品。梯度增强的同核和异核化学位移相关技术用于进行共振归属。梯度NOESY实验可深入了解脂质、布洛芬和水的位置及动力学。梯度的应用不仅减少了实验时间，还降低了多维谱中的t(1)噪声。用脉冲场梯度进行的扩散测量表征了脂质和药物分子在膜中的横向运动。本文提出了关于随机取向的多层脂质体MAS扩散实验数据分析的理论框架。
