Jovanović-Galović A, Blagojević D P, Grubor-Lajsić G, Worland R, Spasić M B
Institute of Biology, Faculty of Science, Novi Sad, Yugoslavia.
Arch Insect Biochem Physiol. 2004 Feb;55(2):79-89. doi: 10.1002/arch.10126.
Antioxidant enzymes, total glutathione (GSH), and ascorbic acid (ASA) were determined in whole body homogenates of nondiapausing larvae, diapausing larvae during the diapausing period (October, December, and February), and in pupae emerged from both diapausing and nondiapausing larvae of the European corn borer (Ostrinia nubilalis, Hubn., Lepidoptera: Pyralidae). The activities of catalase, selenium nondependent glutathione peroxidase (GPx), and glutathione-S-transferase (GST), as well as the content of GSH and ASA, were found to vary throughout the larval diapause. Compared to diapausing larvae, nondiapausing larvae were higher in levels of catalase, GPx, GST, and dehydroascorbate reductase (DHAR) activity. GSH content was also increased. However, nondiapausing larvae contained less ASA than diapausing ones. Pupae had higher GPx and GST activity and an increased ASA content compared to larvae. The pupae emerged from nondiapausing larvae had higher GST, glutathione reductase (GR), and DHAR activities, but lower GPx activity and ASA content than those emerged from diapausing larvae. Correlation analysis revealed differences in the way the antioxidant level is equilibrated for a particular stage and developmental pattern. The results suggest that cellular antioxidants are involved in both the protection of cells and the regulation of redox levels during the pre-adult stages of Ostrinia nubilalis. Arch. Insect Biochem. Physiol. 55:79-89, 2004.
在欧洲玉米螟（Ostrinia nubilalis, Hubn., 鳞翅目：螟蛾科）的非滞育幼虫、滞育期（10月、12月和2月）的滞育幼虫以及由滞育和非滞育幼虫羽化出的蛹的全身匀浆中，测定了抗氧化酶、总谷胱甘肽（GSH）和抗坏血酸（ASA）的含量。结果发现，过氧化氢酶、硒非依赖性谷胱甘肽过氧化物酶（GPx）和谷胱甘肽-S-转移酶（GST）的活性以及GSH和ASA的含量在幼虫滞育期间均有变化。与滞育幼虫相比，非滞育幼虫的过氧化氢酶、GPx、GST和脱氢抗坏血酸还原酶（DHAR）活性水平更高，GSH含量也有所增加。然而，非滞育幼虫的ASA含量比滞育幼虫少。与幼虫相比，蛹的GPx和GST活性更高，ASA含量增加。由非滞育幼虫羽化出的蛹的GST、谷胱甘肽还原酶（GR）和DHAR活性较高，但GPx活性和ASA含量比由滞育幼虫羽化出的蛹低。相关性分析揭示了特定阶段和发育模式下抗氧化水平平衡方式的差异。结果表明，细胞抗氧化剂在欧洲玉米螟成虫前期的细胞保护和氧化还原水平调节中均发挥作用。《昆虫生物化学与生理学档案》55:79 - 89, 2004年。
