Shapira Boaz, Karton Amir, Aronzon Dina, Frydman Lucio
Department of Chemical Physics, Weizmann Institute of Science, 76100 Rehovot, Israel.
J Am Chem Soc. 2004 Feb 4;126(4):1262-5. doi: 10.1021/ja0389422.
We have recently proposed and demonstrated an approach that enables the acquisition of multidimensional nuclear magnetic resonance (NMR) spectra within a single scan. A promising application opened up by this new accelerated form of data acquisition concerns the possibility of monitoring in real time the chemical nature of analytes subject to a continuous flow. The present paper illustrates such potential, with the real-time acquisition of a series of 2D 1H NMR spectra arising from a mixture of compounds subject to a continuous liquid chromatography (LC) separation. This real-time 2D NMR identification of chemicals eluted minutes apart under usual LC-NMR conditions differs from the way in which LC-2D NMR has hitherto been carried out, which relies on stopped-flow modes of operations whereby fractions are first collected and then subject to individual, aliquot-by-aliquot analyses. The real-time LC-2D NMR experiment hereby introduced can be implemented in a straightforward manner using modern commercial LC-NMR hardware, thus opening up immediate possibilities in high-throughput characterizations of complex molecules.
我们最近提出并证明了一种方法，该方法能够在单次扫描中获取多维核磁共振（NMR）光谱。这种新的加速数据采集形式开辟了一个有前景的应用领域，即有可能实时监测连续流动的分析物的化学性质。本文阐述了这种潜力，通过实时采集一系列二维¹H NMR光谱，这些光谱来自经过连续液相色谱（LC）分离的化合物混合物。在通常的LC-NMR条件下，对相隔几分钟洗脱的化学物质进行实时二维NMR鉴定，这与迄今为止进行LC-2D NMR的方式不同，后者依赖于停流操作模式，即首先收集馏分，然后对每个馏分进行逐份分析。本文介绍的实时LC-2D NMR实验可以使用现代商用LC-NMR硬件以直接的方式实现，从而为复杂分子的高通量表征开辟了直接的可能性。
