后期滞后主要解释了人类植入前胚胎中的染色体嵌合现象。

Anaphase lagging mainly explains chromosomal mosaicism in human preimplantation embryos.

作者信息

Coonen Edith, Derhaag Josien G, Dumoulin John C M, van Wissen Lucie C P, Bras Marijke, Janssen Marij, Evers Johannes L H, Geraedts Joep P M

机构信息

Research Institute Growth & Development (GROW), Maastricht University and Department of Obstetrics & Gynaecology, Academic Hospital Maastricht, The Netherlands.

出版信息

Hum Reprod. 2004 Feb;19(2):316-24. doi: 10.1093/humrep/deh077.

DOI:10.1093/humrep/deh077

PMID:14747173

Abstract

BACKGROUND

Cleavage stage embryos as well as postimplantation embryos have been studied extensively over the years. However, our knowledge with respect to the chromosomal constitution of human embryos at the blastocyst stage is still rudimentary.

METHODS

In the present paper, a large series of human blastocysts was examined by means of fluorescent in situ hybridization (FISH).

RESULTS

It was found that only one in four blastocysts (25%) displayed a normal chromosomal pattern. We defined a group of blastocysts (26%) displaying a simple mosaic chromosome pattern (different cell lines resulting from one chromosomal error), an about equally large group of blastocysts (31%) displaying a complex mosaic chromosome pattern, and a smaller group of blastocysts (11%) showing a chaotic chromosome distribution pattern. Six per cent of all blastocysts analysed could not be assigned one of the previously mentioned chromosomal patterns.

CONCLUSION

Anaphase lagging appeared to be the major mechanism through which human embryos acquire a mosaic chromosome pattern during preimplantation development to the blastocyst stage.

摘要

背景

多年来，人们对卵裂期胚胎以及着床后胚胎进行了广泛研究。然而，我们对人类囊胚期胚胎染色体构成的了解仍然很基础。

方法

在本文中，通过荧光原位杂交（FISH）对大量人类囊胚进行了检测。

结果

发现只有四分之一的囊胚（25%）显示出正常的染色体模式。我们定义了一组囊胚（26%）呈现简单的嵌合染色体模式（由一个染色体错误导致不同细胞系），数量大致相同的另一组囊胚（31%）呈现复杂的嵌合染色体模式，以及一小部分囊胚（11%）显示出混乱的染色体分布模式。所有分析的囊胚中有6%无法归为上述任何一种染色体模式。