Sallam Lotfy A R, El-Refai Abdel-Monem H, Hamdy Abdel-Hamid A, El-Minofi Hassaan A, Abdel-Salam Ibrahim S
Microbial and Natural Products Chemistry Department, National Research Centre, Dokki, Cairo, Egypt.
J Gen Appl Microbiol. 2003 Dec;49(6):321-8. doi: 10.2323/jgam.49.321.
A local isolate of Aspergillus terreus was selected among different microorganisms as a new cyclosporin A (Cy A) producing culture. The formation of Cy A was investigated under different fermentation conditions (including selection of the cultivation medium, fermentation time course, inoculum nature, medium volume, agitation rate, pH value). Relatively high Cy A productivities were maintained when the fermentation process was carried out using a medium composed of (g/L): glucose, 50; bactopeptone, 10; KH(2)PO(4), 5; KCl, 2.5; pH 5.3, inoculated with 2% standard inoculum of 48 h age, shaken at 200 rpm for 10 days.
从不同微生物中挑选出一株土曲霉的本地分离株，作为一种新的产生环孢素A（Cy A）的培养物。在不同发酵条件下（包括培养基的选择、发酵时间进程、接种物性质、培养基体积、搅拌速率、pH值）研究了Cy A的形成。当使用由（克/升）组成的培养基进行发酵过程时，能保持相对较高的Cy A生产率：葡萄糖50；细菌蛋白胨10；KH(2)PO(4) 5；KCl 2.5；pH 5.3，接种2%的48小时龄标准接种物，以200转/分钟振荡培养10天。
