Sallam Lotfy A R, El-Refai Abdel-Monem H, Hamdi Abdel-Hamid A, El-Minofi Hassaan A, Abd-Elsalam Ibrahim S
Microbial and Natural Products Chemistry Department, National Research Centre, Cairo, Egypt.
J Gen Appl Microbiol. 2005 Jun;51(3):143-9. doi: 10.2323/jgam.51.143.
The formation of cyclosporin A (Cy A) by immobilized spores and mycelia of Aspergillus terreus was investigated. Different carriers were tested as immobilizing carriers, whereby Ca-alginate was selected for further experimentation. The role of alginate concentration, biomass weight, pH value of the cultivation medium, repeated utilization of the immobilized fungus as well as the supplementation of different amino acid precursors were studied. Best Cy A outputs were attained with Ca-alginate 3% (w/v), mycelial weight 15% (w/v), pH 4.5 and four repeated cycles. Similarly, the Cy A productivity was markedly accelerated in the presence of L-valine and L-valine and L-leucine mixture.
研究了土曲霉固定化孢子和菌丝体合成环孢菌素A（Cy A）的情况。测试了不同载体作为固定化载体，从而选择海藻酸钙进行进一步实验。研究了海藻酸钠浓度、生物量重量、培养基pH值、固定化真菌的重复利用以及不同氨基酸前体的添加所起的作用。使用3%（w/v）的海藻酸钙、15%（w/v）的菌丝体重量、pH 4.5以及四个重复循环可获得最佳的Cy A产量。同样，在L-缬氨酸以及L-缬氨酸和L-亮氨酸混合物存在的情况下，Cy A的生产率显著提高。
