Surrallés J, Carbonell E, Marcos R, Degrassi F, Antoccia A, Tanzarella C
Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Mutagenesis. 1992 Nov;7(6):407-10. doi: 10.1093/mutage/7.6.407.
A collaborative study between two laboratories has been carried out in order to investigate the reproducibility of the cytokinesis-block micronucleus assay in human lymphocytes. A modified protocol to obtain good quality slides from whole blood cultures has been developed. The spontaneous frequencies of micronuclei in binucleated lymphocytes from five male subjects were evaluated in each laboratory at two cytochalasin-B concentrations (3 and 6 micrograms/ml). Our data show that 6 micrograms/ml cytochalasin-B was more effective than 3 micrograms/ml to arrest cytokinesis in all the donors analysed. The mean frequency of micronuclei in binucleated cells was significantly lower at 6 micrograms/ml compared to 3 micrograms/ml cytochalasin-B in both laboratories. The results obtained indicate a very high concordance between both sets of data supporting the validity of the proposed protocol.
为了研究人类淋巴细胞中胞质分裂阻滞微核试验的可重复性，两个实验室开展了一项合作研究。已开发出一种改良方案，用于从全血培养物中获得高质量的玻片。在每个实验室中，使用两种细胞松弛素B浓度（3微克/毫升和6微克/毫升）评估了五名男性受试者双核淋巴细胞中的微核自发频率。我们的数据表明，在所有分析的供体中，6微克/毫升的细胞松弛素B比3微克/毫升更有效地阻滞胞质分裂。在两个实验室中，与3微克/毫升的细胞松弛素B相比，6微克/毫升时双核细胞中的微核平均频率显著更低。所获得的结果表明两组数据之间具有非常高的一致性，支持了所提议方案的有效性。
